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Screening Study of Influence of Selected Alkaloids from Eschscholtzia Californica Cham. (Papaveraceae) on Human Erythrocytal Acetylcholinesterase
Opletal, L. ; Cahlíková, L. ; Dohnal, V. ; Kurfürst, Milan ; Schraml, Jan ; Jun, D. ; Musilová, L. ; Doležal, J. ; Jahodář, L.
Primary extract was prepared by extraction of whole plant with 95% EtOH and then secondary extracts were made. From ethereal extract (pH 9-10) benzofenantridin alkaloids were separated, remaining tertiary bases were divided according to solubility of their chlorides in chloroform; thus, phenolic and non-phenolic alkaloids were obtained from each fraction.
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Influence of Isochinolinic A-Fraction (Phenolic CHCl3 Insoluble Cl- > Salts) Alkaloids from Corydalis Cava(Fumariaceae) on Cholinesterases
Chlebek, J. ; Musilová, L. ; Jun, D. ; Opletal, L. ; Kurfürst, Milan ; Schraml, Jan ; Dohnal, V. ; Jahodář, L.
From 12 kg of Corydalis cava bulbs, ethanolic extract was prepared. From this extract, pure alkaloid concentrates. Tertiary bases of average basicity (extract A, 200g) were disolved in hydrochloric acid and two fractions were separated, based on solubility in chloroform. Both fractions were further resoluted to phenolic and non-phenolic bases. Their structure was determined by MS and NMR. Effectivity of obtained compounds was evaluated in vitro, or determined by standard IC50 inhibition test. Human erythrocytal AChE and serum BuChE were used as source ov AChE. Activity was measured spectrophotometrically using standard Elman method. Results were compared to values of IC50 of reference compounds – terapeutic inhibitors (donepezil, rivastigmin, galanthamin, huperzin A).
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