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Localisation of FUBP3 protein in human cervical cancer cell line
Kunrt, Josef ; Jirkovský, Eduard (advisor) ; Macháček, Miloslav (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Candidate: Josef Kunrt Supervisor: Eduard Jirkovský, PharmD, PhD.; Assist. Prof. Nika Lovšin, M.Chem., PhD Title of Diploma Thesis: Localisation of FUBP3 Protein in Human Cervical Cancer Cell Line Osteoporosis is a skeletal disorder characterised by an impaired bone mass, strength and microarchitecture which leads to a higher risk of fracture. The disease is predominant in postmenopausal women but also affects older men and other risk groups, such as oncological patients. To ascertain the genetic basis of osteoporosis, multiple genome-wide association studies have been performed with the aim to identify genes with a role in the diseases. These studies discovered multiple genetic loci associated with a higher risk of osteoporotic fractures, one of which was far upstream element-binding protein 3 (FUBP3). Works studying the properties of this protein such as cellular localisation are scarce. Therefore, we focused on establishing a protocol for visualisation of FUBP3 and its co-localization with other cellular structures in a human cervical cancer cell line (HeLa). The visualisation was done using immunostaining of FUBP3 and GM130, a matrix protein of Golgi apparatus, and subsequent imaging with...
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Problems connected with the application of lamella flanges in steel bridge construction
Křístek, V. ; Kunrt, J. ; Škaloud, Miroslav ; Urushadze, Shota
Lamella flanges have lately grown very popular with the designers of steel bridges, because - in their belief - they provide us with the possibility of avoiding very thick flange plates in steel bridge structures. This belief is based on the assumption that the lamellas are perfectly plane and therefore, in perfect contact everywhere, so that the loading from one lamela is transmitted into other via pure compression, and that the perfect interaction of both lamellas is materialized by means of boundary fillet welds connecting both of the two lamellas. This simple assumption is, however, far from reality: it is not the means of steel fabricators, not even in the means of those which are very progressively equipped, to produce perfectly plane lamellas. Then both lamellass exhibit unavoidable initial curvatures, which in combination form a gap between the lamellas, and consequently the directly loaded lamela are pressed into this gap. As the loading acting on every bridge is many times repeated, the aforsaid phenomenon is also many times repeated, (we can say that the lamellas "breathe"), and then an unavoidable cumulative damage proces in the lamellas comes to being. The results of investigation will be described in the paper and thereby will demonstrate that the fatigue phenomenon accompanying the breathing of the lamellas can considerably limit the lifetime of the structure.
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The breathing of the lamella flanges of the Lochkov Bridge on SOKP
Kunrt, J. ; Křístek, V. ; Škaloud, Miroslav
The main results obtained by an extended test on the fatigue behaviour generated by changes in the relative distance of the plate elements of the lamella flanges of the Lochkov Bridge, which occur under the passages of heavy vehicles are presented. The plate elements exhibit unavoidable out-of-plane imperfections, which results in them not being in perfect contact. The lamellas are then subjected to many times repeated bending, which generates cumulative damage in the welds that connect the two lamellas.
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