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Physiological and neurobiological correlates of personality traits
Kubelková, Klára ; Nekovářová, Tereza (advisor) ; Jonáš, Juraj (referee)
This paper seeks to provide an overview of the molecular, genetic, as well as structural and functional correlates of personality traits known to date, focusing primarily on typology according to the extensively used "NEO PI-R" ("Big Five") classification. It also attempts to discuss, at least briefly, the methodology for obtaining both neurobiological and psychological data and its validity. Finally, it aims to highlight the strengths and weaknesses of the neurobiological approach to personality, its potential benefits and pitfalls. It does not aim to establish the exact proportion of biological and sociological factors, but to elucidate the mechanisms involved in this biological conditioning. Although there is now a vast amount of research on the biological substrate of personality, there is considerable inconsistency among these studies, both in terms of methodology and the results provided. Moreover, only a small number of them attempt to explain their findings in terms of possible mechanisms of their effect and thus to integrate them into the broader picture of the brain-personality connection. These and many other limitations of neurobiological research on personality, which receive considerable attention in this thesis, are only a proof of the immense complexity of the human brain and human...
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Entry of bacteria Mycobacterium bovis BCG into B lymphocytes
Šamajová, Marianna ; Konečná, Klára (advisor) ; Kubelková, Klára (referee)
Charles University Faculty of Pharmacy in Hradec Králové Study program: Pharmacy Author: Marianna Šamajová Supervisor: RNDr. Klára Konečná, Ph.D. Consultant: plk. gšt. doc. RNDr. Zuzana Kročová, Ph.D. Title of diploma thesis: Entry of bacteria Mycobacterium bovis BCG into B lymphocytes Background: The objective of this work was to evaluate the entry of bacterium Mycobacterium bovis BCG into B lymphocytes and the role of selected receptors in this process. Methods: Peritoneal cell suspensions with unblocked and/or blocked receptors on BALB/c mouse B lymphocytes we infected by bacterium M. bovis BCG-GFP unopsonized and/or opsonized by fresh murine serum ("complement") or immune serum ("antibodies"). Using flow cytometry we evaluated the entry of bacterium M. bovis BCG-GFP into B lymphocytes and their subpopulations B1a, B1b and B2. Results: M. bovis BCG-GFP actively enters into B lymphocytes. Depending on the subpopulation, it most infects B1a, less B1b and at least B2 lymphocytes. Only the subpopulation B2 responds significantly to the opsonization by complement. Opsonization by antibodies had no significant effect on the infection. Entry into CD19+ cells is mediated through the BCR receptor, especially in subpopulations B1a and B1b. Under the opsonized conditions, the CR1/2 complement receptor is...
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Entry of bacteria Mycobacterium bovis BCG into B lymphocytes
Šamajová, Marianna ; Konečná, Klára (advisor) ; Kubelková, Klára (referee)
Charles University Faculty of Pharmacy in Hradec Králové Study program: Pharmacy Author: Marianna Šamajová Supervisor: RNDr. Klára Konečná, Ph.D. Consultant: plk. gšt. doc. RNDr. Zuzana Kročová, Ph.D. Title of diploma thesis: Entry of bacteria Mycobacterium bovis BCG into B lymphocytes Background: The objective of this work was to evaluate the entry of bacterium Mycobacterium bovis BCG into B lymphocytes and the role of selected receptors in this process. Methods: Peritoneal cell suspensions with unblocked and/or blocked receptors on BALB/c mouse B lymphocytes we infected by bacterium M. bovis BCG-GFP unopsonized and/or opsonized by fresh murine serum ("complement") or immune serum ("antibodies"). Using flow cytometry we evaluated the entry of bacterium M. bovis BCG-GFP into B lymphocytes and their subpopulations B1a, B1b and B2. Results: M. bovis BCG-GFP actively enters into B lymphocytes. Depending on the subpopulation, it most infects B1a, less B1b and at least B2 lymphocytes. Only the subpopulation B2 responds significantly to the opsonization by complement. Opsonization by antibodies had no significant effect on the infection. Entry into CD19+ cells is mediated through the BCR receptor, especially in subpopulations B1a and B1b. Under the opsonized conditions, the CR1/2 complement receptor is...
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Entry of bacteria Mycobacterium bovis BCG into B lymphocytes
Šamajová, Marianna ; Konečná, Klára (advisor) ; Kubelková, Klára (referee)
Charles University Faculty of Pharmacy in Hradec Králové Study program: Pharmacy Author: Marianna Šamajová Supervisor: RNDr. Klára Konečná, Ph.D. Consultant: plk. gšt. doc. RNDr. Zuzana Kročová, Ph.D. Title of diploma thesis: Entry of bacteria Mycobacterium bovis BCG into B lymphocytes Background: The objective of this work was to evaluate the entry of bacterium Mycobacterium bovis BCG into B lymphocytes and the role of selected receptors in this process. Methods: Peritoneal cell suspensions with unblocked and/or blocked receptors on BALB/c mouse B lymphocytes we infected by bacterium M. bovis BCG-GFP unopsonized and/or opsonized by fresh murine serum ("complement") or immune serum ("antibodies"). Using flow cytometry we evaluated the entry of bacterium M. bovis BCG-GFP into B lymphocytes and their subpopulations B1a, B1b and B2. Results: M. bovis BCG-GFP actively enters into B lymphocytes. Depending on the subpopulation, it most infects B1a, less B1b and at least B2 lymphocytes. Only the subpopulation B2 responds significantly to the opsonization by complement. Opsonization by antibodies had no significant effect on the infection. Entry into CD19+ cells is mediated through the BCR receptor, especially in subpopulations B1a and B1b. Under the opsonized conditions, the CR1/2 complement receptor is...
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. Changes in gene expression of B cell line Ramos RA-1 after infection with Francisella tularensis.
Matyášová, Simona ; Konečná, Klára (advisor) ; Kubelková, Klára (referee)
Francisella tularensis is a facultative intracellular bacterium causing disease tularemia of different species of mammals, including humans. It is able to infect phagocytotic as well as non-phagocytotic cells. We focused on the study of B cells, that role in the infection of F. tularensis have been, until recently, regarded as not significant. Over the last 10 years several studies showed important role of B cells in innate immunity to F.tularensis infection. The aim of this thesis was to idenify changes in the expression of genes that encode molecules of cytokines produced by B cells, after F. tularensis infection, due to stimulation with bacterial recombinant protein IglC and lipopolysaccharide. These changes were examinated on B cell line Ramos RA-1. Using reverse transcriptase polymerase chain reaction, we detected these cytokines: IFN-γ, IL-10 a TGF-β. Key words: Francisella tularensis, B cells, cytokines, reverse transcriptase polymerase chain reaction
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