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Role of Zinc-α2-glycoprotein in tumors
Kotvalová, Hanka ; Šmahel, Michal (advisor) ; Kubů, Martin (referee)
The Zinc-α2-glycoprotein (ZAG) encoded by the AZGP1 gene particularly contributes to the regulation of lipid and glucose metabolism. In different types of tumors, its altered production was found and the association of the ZAG level with the patient's prognosis was suggested. However, the role of ZAG in tumor progression can differ in individual malignancy types and mechanisms of ZAG function are not sufficiently identified. The objectives of the bachelor's thesis are a description of the structure of the ZAG protein and the regulation of AZGP1 expression, a summary of the identified ZAG functions in nonmalignant diseases, and a focus on the role of ZAG in tumors, particularly on the mechanisms of its action and its potential use as a biomarker.
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Effect of ADAR1 enzyme on hepatitis C virus life cycle
Kubů, Martin ; Vopálenský, Václav (advisor) ; Fraiberk, Martin (referee)
Hepatitis C virus (HCV) is a virus of the family Flaviviridae whose genome consists of ,,+RNA" molecule. It causes the disease hepatitis Cepatitida typu C, which affects tens of millions of people worldwide. Although there is an effective treatment for this type of hepatitis, a preventive vaccine against the HCV virus has not yet been developed. Several ambigious works focused on the relationship between HCV and the enzyme adenosine deaminase acting on double-stranded RNA 1 (ADAR1) were published in the past. This dimeric double-stranded RNA binding enzyme is a part of innate immunity and causes catalytic conversion of adenosine to inosine, which is recognized by cellular mechanisms as guanine, which ultimately leads to mutations in the affected dsRNA molecule. The works published so far attribute an antiviral function to the ADAR1 enzyme in the context of HCV infection. However, vectors containing the entire HCV genome were not used in these works, and a cell line with deletion od the ADAR1 gene has never been used so far. The actual relationship between the ADAR1 enzyme and the HCV virus has not yet been reliably verified. The aim of this thesis was to gain a deeper understanding of the relationship between the HCV virus and the ADAR1 enzyme. For this task, HCV permissive Huh7.5 cell lines with...
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Effect of adenosine deaminase acting on RNA on viral infection of eukaryotic cells
Kubů, Martin ; Vopálenský, Václav (advisor) ; Fraiberk, Martin (referee)
Double-stranded RNA is a molecule rarely found in a cell, but it is specific for viral infection. It is also a substrate of ADAR enzymes. These enzymes convert adenosin to inosine, which is recognized as guanosine by cellular machinery. Apart from editing activity, ADAR enzymes interact with cellular proteins, such as Dicer and protein kinase R, which together with editing affects viral replication. In this work, the information about antiviral activity of ADAR enzymes and their impact on infection of selected primarily human viruses is reviewed.
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Balance of import and export of foodstuffs CZ-Poland in the last decade (focused on meat products)
Hrubá, Andrea ; Olšanová, Květa (advisor) ; Kubů, Martin (referee)
The bachelor thesis is focused on import and export balance of foodstuffs, mainly meat products, between the Czech Republic and Poland in years 2003 -- 2013. It analyses foreign trade with foodstuffs, especially with meat products of these countries and evaluates ascertained data in terms of time span of ten years. It describes the effect of major events on the imports and exports. They are: The entry of both countries into the European Union in 2004, the global financial crisis in 2008 and problems with the quality of foodstuff originating from Poland in year 2013. The aim of the bachelor thesis is to map, evaluate and summarize ascertained data and highlight the results and consequences.
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