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Criteria for drinking water for breeding medical leeches
Krýslová, Jana ; Řezáčová, Veronika (referee) ; Komendová, Renata (advisor)
This bachelor thesis deals with the issue of breeding leeches with regard to the quality of the water in which it is bred. It focuses on the comparison of individual criteria of drinking water taken from different sources of drinking water (Letovice, Brno and the surroundings of Brno). The sources of these waters are from Vír and Březová, or they are mixed in a certain ratio. Selected indicators of drinking water quality were measured in individual water samples using UV-VIS spectrometry, flame photometry, turbidimetry and volumetric methods. Rajec baby water, purchased from the retail network, was selected as a comparative sample. From the obtained data it was found that the values of water hardness have the greatest influence on the success of leech breeding. It is generally known that too hard water does not benefit the leech, and therefore it dies. For the other determined parameters, it was not possible to prove whether they have a significant influence on the breeding of leeches and its death.
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Involvement of various gelation strategies in development of next-generation bioinnoculants
Krýslová, Jana ; Smilek, Jiří (referee) ; Sedláček, Petr (advisor)
This diploma thesis deals with the investigation cross-linking approaches of the bacterium Azotobacter vinelandii, which belongs to the PGPR group and is capable of producing alginate extracellularly. This crosslinking concept represents a novel approach to development of bioinoculants based on the PGPR. The first part is dedicated to investigating the effect of oxygen on the gelation of bacterial cultures itself. Experimentally, it was found that bacterial cultures in a total volume of 50 and 100 ml in 250 ml Erlenmeyer flasks showed spontaneous gelation already during the cultivation process, while the formed gels were measured rheometrically and showed values of const. complex modulus 0,026 kPa and 0,030 kPa. With a bacterial culture in a total volume of 150 ml, spontaneous cross-linking itself did not occur. In addition to gelation testing, the content of PHB and extracellular alginate and its molecular weight were determined in the bacterial cultures using size exclusion chromatography with multi-angle light scattering (SEC-MALS) and the mechanical properties of the resulting gels using rheometry. Cross-linking of bacterial cultures took place mainly with the use of CaCl2 as a cross-linking agent, but various alternative methods of gelation were tested as well (by means of polyvalent ions, weak organic acids, precipitants such as DMSO, emulsion gelation). The effect of glucono-D-lactone (GDL) as a gelling agent based on a slow release of crosslinking ions from their insoluble form was also tested. The strongest gels were formed by the addition of polyvalent ions (they ranged in the value of the complex modulus of 10 kPa, in the case of the use of weak acids and DMSO, the gels ranged in the value of the complex modules in the range of 1 to 10 kPa. The addition of GDL did not create gels immediately, but delayed, and gelation was observed in time.The formed gel ranged in value up to 1 kPa.
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Criteria for drinking water for breeding medical leeches
Krýslová, Jana ; Řezáčová, Veronika (referee) ; Komendová, Renata (advisor)
This bachelor thesis deals with the issue of breeding leeches with regard to the quality of the water in which it is bred. It focuses on the comparison of individual criteria of drinking water taken from different sources of drinking water (Letovice, Brno and the surroundings of Brno). The sources of these waters are from Vír and Březová, or they are mixed in a certain ratio. Selected indicators of drinking water quality were measured in individual water samples using UV-VIS spectrometry, flame photometry, turbidimetry and volumetric methods. Rajec baby water, purchased from the retail network, was selected as a comparative sample. From the obtained data it was found that the values of water hardness have the greatest influence on the success of leech breeding. It is generally known that too hard water does not benefit the leech, and therefore it dies. For the other determined parameters, it was not possible to prove whether they have a significant influence on the breeding of leeches and its death.
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