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The effect of pyrazine derivatives on secondary metabolites in in vitro cultures
Kovalíková, Zuzana ; Tůmová, Lenka (advisor) ; Kašparová, Marie (referee)
Vplyv derivátov pyrazínu na tvorbu sekundárnych metabolitov v rastlinných kultúrach in vitro - Zuzama Kovalíková - 71 - 11 Abstract Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmacognosy Candidate: Mgr. Zuzana Kovalíková Consultant: doc. PharmDr. Lenka Tůmová, CSc. Title of Thesis: The effect of pyrazine derivatives on secondary metabolites in in vitro cultures. This thesis investigates the effect of new synthetic pyrazine derivatives as potential elicitors on rutin production in callus and suspension cultures of Fagopyrum esculentum, var. Bambi. All tested chemical elicitors increased the production of rutin in both callus and suspension cultures. Callus and suspension culture was cultivated in Murashige and Skoog nutrient medium containing growth regulator (2,4-D) under the specified temperature and light conditions. The samples were taken in 6, 12, 24, 48, 72 and 168 hours after elicitor exposition, control samples were taken in 24 and 168 hours after exposition. The determination of the rutin content was performed by high performance liquid chromatography (HPLC). Elicitor P.G.R.1, propyl 5-(3-(4.chlorophenyl)ureido)pyrazine-2-carboxylate showed the highest increase in rutin production after 12 hours treatment in the callus culture, whereas the highest increase in...
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The effect of pyrazinederivatives on secondarymetabolitesproductionin in vitro cultures
Klepalová, Libuše ; Tůmová, Lenka (advisor) ; Kovalíková, Zuzana (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmacognosy Candidate Mgr. Libuše Klepalová Consultant Doc. PharmDr. Lenka Tůmová CSc. Title of Thesis The effect of pyrazine derivatives on secondary metabolites production in in vitro cultures This work studies the possibility of influencing the production of silymarin and taxifoline in in vitro cultures of Silybum marianum L. using the elicitation method. The pyrazine derivate BBPS3 in three different concentrations (c1-3.3529·10-3 mol/l, c2-3.3529 ·10-4 mol/l and c3-3.3529·10-5 mol/l) was used as elicitor. Sampling took place at 6, 24, 48, 72 and 168 hours after application of the substance. Control samples were left without the use of an elicitor. At the same time, samples of the nutrient medium were taken. The analysis was performed using the HPLC method. Thus, in order to increase the content of silymarin complex and taxifoline, it seemed more appropriate to use a callus culture, compared to a suspension culture. The statistically most significant increase in the content of silymarin complex in callus culture was achieved after 72 h of elicitor treatment with a concentration of c2 (80.7 µg/g DW). Statistically the most significant increase in the taxifoline content occurred after 168 h of elicitation of the...
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Role of carbohydrate metabolism in defence against oxidative stress induced by arsenic
Kofroňová, Monika ; Lipavská, Helena (advisor) ; Száková, Jiřina (referee) ; Kovalíková, Zuzana (referee)
Heavy metal contamination significantly reduces crop yields, causing serious problems in agriculture and having a major impact on human health if these contaminants enter the food chain. Understanding the mechanisms of plant responses could help to increase their resistance to heavy metals as well as their potential use in phytoremediation. Carbohydrates play an important role in plant growth and development as well as in defense reactions. This work summarizes the results of four publications focused on the effects of arsenic and thorium on antioxidant mechanisms in tobacco plants and horseradish root crops. Attention is paid, among other things, to the dynamics of sugar contents, which are potentially important molecules involved in the fight against oxidative stress. The first publication summarizes arsenic effects on plant physiological parameters, focusing on arsenic tolerance-enhancing mechanisms as well as summarizing the ability of plants to cope with arsenic-induced oxidative and nitrosative stress. Emphasis was placed on, among other things, a topic that was unjustly neglected in previous publications - i.e. carbohydrate metabolism. Further work was already experimental and dealt with the study of arsenic as a trigger of oxidative stress in the root culture of horseradish and...
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The effect of pyrazine derivatives on secondary metabolites in in vitro cultures
Kovalíková, Zuzana ; Tůmová, Lenka (advisor) ; Kašparová, Marie (referee)
Vplyv derivátov pyrazínu na tvorbu sekundárnych metabolitov v rastlinných kultúrach in vitro - Zuzama Kovalíková - 71 - 11 Abstract Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmacognosy Candidate: Mgr. Zuzana Kovalíková Consultant: doc. PharmDr. Lenka Tůmová, CSc. Title of Thesis: The effect of pyrazine derivatives on secondary metabolites in in vitro cultures. This thesis investigates the effect of new synthetic pyrazine derivatives as potential elicitors on rutin production in callus and suspension cultures of Fagopyrum esculentum, var. Bambi. All tested chemical elicitors increased the production of rutin in both callus and suspension cultures. Callus and suspension culture was cultivated in Murashige and Skoog nutrient medium containing growth regulator (2,4-D) under the specified temperature and light conditions. The samples were taken in 6, 12, 24, 48, 72 and 168 hours after elicitor exposition, control samples were taken in 24 and 168 hours after exposition. The determination of the rutin content was performed by high performance liquid chromatography (HPLC). Elicitor P.G.R.1, propyl 5-(3-(4.chlorophenyl)ureido)pyrazine-2-carboxylate showed the highest increase in rutin production after 12 hours treatment in the callus culture, whereas the highest increase in...
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