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National Repository of Grey Literature 3 records found  Search took 0.01 seconds. 
Zamestnanecké benefity v sektore obchodných firiem
 Kováčová, Kristína
The thesis focuses on one of the forms of motivation and remuneration of employees in Slovakia – employee benefits. Benefits are discussed in terms of usability and value, but the tax impact on employees and their employers is essential. This tax incidence is realized by valid legal regulations. Benefit usability is depicted on a particular Slovak business company as a demonstration of practice. Using the questionnaire survey, it examined the satisfaction of employees with their benefits. The results have led to the proposal of a new benefit structure for a company that has been economically quantified.
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Preparation of fluorescently labeled substrates and determination of transglycosylation activity in plants
 Velínská, Kamila ; Ing.Kristína Kováčová (referee) ; Firáková, Zuzana (advisor)
The bachelor's thesis was focused on preparation of fluorescently labeled oligosaccharides and detection of transglycosylating/heterotransglycosylating activities of enzymes from dicotyledons. As the model organisms nasturtium and parsley were used. The quality and composition of labeled acceptor substrates was monitored by TLC and MALDI-TOF MS. The presence of enzymes in the plant seeds was analysed by SDS electrophoresis and IEF-PAGE. The paper method and HPLC were used for the detection of transglycosylating activities. The obtained results suggest that enzymes catalyzing heterotransglycosylating reactions are in the seeds of studied plants.
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Expression and characterization of Phr2 protein of Candida albicans
 Vlčková, Zuzana ; Ing.Zuzana Firáková (referee) ; Kováčová, Kristína (advisor)
Transglycosylases have an import function in yeast the cell wall. These enzymes cleave a molecule of donor, which is followed by transferring a newly created reducing end to a non-reducing end of a molecule of an acceptor. This process enables a growth, expansion and shaping of the yeast cell walls during their life. The aim of this Bachelor Thesis is to characterise the role of Phr2 recombinant protein of Candida albicans in a transglycosylase activity. Using fluorescent paper method, optimal pH and optimal temperature of transglycosylase activity of Phr2 protein were found. The same method was used to establish the acceptor and donor specificity of Phr2p was performed as another method for substrate specificity measurement. High donor and acceptor specificity of Phr2 protein to -1,3-glucan was confirmed. HPLC products of transglycosylase reaction catalysed by Phr2p with laminarin as a donor and L5-SR as an acceptor containing a fluorescent marker were detected by TLC and MALDI-TOF.
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