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National Repository of Grey Literature 3 records found  Search took 0.01 seconds. 
Study of Radiolabelling DOTA-Sargastrin and Determination the Stability of Labeled Product
 Korec, David ; Lázníčková, Alice (advisor) ; Trejtnar, František (referee)
Doctoral thesis David Korec Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Biophysics and Physical Chemistry In this doctoral thesis was to find optimal conditions for radiolabelling of DOTA-peptide Sargastrin with metallic radionuclides 111 In or 177 Lu in such a quality that the labeled peptides can also be used at radiopharmacie department for biological experiments. Minimum required radiochemical purity for these experiments was 97%. Modified peptide-DOTA Sargastrin can be succesfully labeled with lutetium-177 with a radiochemical purity greater than 99% at pH 4.52, and heating at 80řC for 20 minutes. In the same way was DOTA- Sargastrin labeled with indium-111 with a maximum achieved radiochemical purity of 98.7%. It was found that a peptide labeled with lutetium showed slightly higher lipophilicity as its elution time was about 20 seconds longer than the elution time of the peptide labeled with indium. It is probably due to the different coordination prperties of both metals. In studying the stability it was shown that the radiochemical purity of 177 Lu-DOTA- Sargastrin remained greater than 99% up to 48 hours when stored in the fridge. The purity of 111 In-DOTA-Sargastrin withstand in the specified limit purity approximately 1.5hours after preparation. The...
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Study of Radiolabelling DOTA-Sargastrin and Determination the Stability of Labeled Product
 Korec, David ; Lázníčková, Alice (advisor) ; Trejtnar, František (referee)
Doctoral thesis David Korec Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Biophysics and Physical Chemistry In this doctoral thesis was to find optimal conditions for radiolabelling of DOTA-peptide Sargastrin with metallic radionuclides 111 In or 177 Lu in such a quality that the labeled peptides can also be used at radiopharmacie department for biological experiments. Minimum required radiochemical purity for these experiments was 97%. Modified peptide-DOTA Sargastrin can be succesfully labeled with lutetium-177 with a radiochemical purity greater than 99% at pH 4.52, and heating at 80řC for 20 minutes. In the same way was DOTA- Sargastrin labeled with indium-111 with a maximum achieved radiochemical purity of 98.7%. It was found that a peptide labeled with lutetium showed slightly higher lipophilicity as its elution time was about 20 seconds longer than the elution time of the peptide labeled with indium. It is probably due to the different coordination prperties of both metals. In studying the stability it was shown that the radiochemical purity of 177 Lu-DOTA- Sargastrin remained greater than 99% up to 48 hours when stored in the fridge. The purity of 111 In-DOTA-Sargastrin withstand in the specified limit purity approximately 1.5hours after preparation. The...
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Stability studies of monoclonal antibody nimotuzumab modified with chelator DTPA radiolabelled with lutetium-177 ([177Lu]Lu-hR3(p-SCN-Bn)DTPA)
 Korec, David ; Kubíček, Vladimír (referee) ; Lázníčková, Alice (advisor)
Stability studies of monoclonal antibody nimotuzumab modified with chelator DTPA radiolabelled with lutetium-177 ([177Lu]Lu-hR3(p-SCN-Bn)DTPA) Diploma thesis David Korec Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Biophysics and Physical Chemistry In this work was target to study the stability of monoclonal antibody Nimotuzumab, which was modified with chelator DTPA radiolabelled with radioactive Lutetium-177 ([177Lu] Lu-hR3 (p- SCN-Bn) DTPA). The radiochemical purity of the supplied antibody was 61%. For other uses had to purified. The chelator EDTA adds in addition for the labeled antibody. It has lower stability constant than the original chelating agent. He can catch the free ligand, which is excreted through the kidneys. For biodistribution studies is needed to ensure the highest purity that was well described by the distribution of radiopharmaceuticals in small bodies. Purification was used for gel permeation chromatography Sephadex. Purity was about 99%. HPLC analysis after one hour showed a small percentage of low molecular weight forms. It may be modified ligand with radionuclide or free radionuclide. At times long after purification the HPLC profile of low molecular weight fraction appeared in two peaks. They are therefore both options possible. The...
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