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Role and regulation of nuclear membrane during meiotic maturation of mammalian oocyte
Končická, Markéta ; Kubelka, Michal (advisor) ; Petr, Jaroslav (referee) ; Binarová, Pavla (referee)
Meiotic division of a female germ cell, an oocyte, is more prone to segregation errors and consequently to aneuploidies than meiosis of a sperm. Aneuploidies and chromosomal aberrations in oocytes increase with higher maternal age in humans and also in mice. Meiotic maturation onset is connected with activity of cyclin dependent kinase 1 (CDK1) that leads to dissociation of nuclear membrane. Moreover regulation of translation of key transcripts is necessary for proper meiotic progression. In thesis findings from four scientific publications are interpreted. We have analyzed the timing of nuclear envelope breakdown (NEBD) and polar body extrusion in mouse oocytes originating from two distinct female age groups: young (2 months old) and aged (12 months old). We found that meiotic maturation happens faster in aged females' oocytes due to early phosphorylation of Lamin A/C, a component of nuclear lamina, and rapid dissociation of nuclear membrane. Moreover aged females' oocytes presented unique characteristic invaginations of nuclear membrane and thus significantly increased circumference of the nuclear envelope compared to the oocytes from young females. These data combined with increased activity of CDK1 and Cyclin B, as well as increased translation of factors that regulate the translation itself,...
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Role and regulation of nuclear membrane during meiotic maturation of mammalian oocyte
Končická, Markéta ; Kubelka, Michal (advisor) ; Petr, Jaroslav (referee) ; Binarová, Pavla (referee)
Meiotic division of a female germ cell, an oocyte, is more prone to segregation errors and consequently to aneuploidies than meiosis of a sperm. Aneuploidies and chromosomal aberrations in oocytes increase with higher maternal age in humans and also in mice. Meiotic maturation onset is connected with activity of cyclin dependent kinase 1 (CDK1) that leads to dissociation of nuclear membrane. Moreover regulation of translation of key transcripts is necessary for proper meiotic progression. In thesis findings from four scientific publications are interpreted. We have analyzed the timing of nuclear envelope breakdown (NEBD) and polar body extrusion in mouse oocytes originating from two distinct female age groups: young (2 months old) and aged (12 months old). We found that meiotic maturation happens faster in aged females' oocytes due to early phosphorylation of Lamin A/C, a component of nuclear lamina, and rapid dissociation of nuclear membrane. Moreover aged females' oocytes presented unique characteristic invaginations of nuclear membrane and thus significantly increased circumference of the nuclear envelope compared to the oocytes from young females. These data combined with increased activity of CDK1 and Cyclin B, as well as increased translation of factors that regulate the translation itself,...
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Life of mothers with children in a shelter.
KONČICKÁ, Markéta
This thesis deals with the life of mothers with children in the shelter. The thesis maps expectations of these women about living in the shelter in the confrontation with their real experience. The thesis is divided into several parts. Theoretical part consists of nine subchapters where the basic concepts related to the issue of social services - shelters, unfavorable social situation, social exclusion and social inclusion are defined. The thesis describes the principles of the activity of shelters for mothers with children, deals with the state of care for mothers with children in our country and characterizes the development and changes in the function of the family. The thesis also deals with the reasons leading to the loss of housing, the issues of homelessness and housing accessibility. It describes and characterizes the possibilities of social protection of mothers with children without home, besides social services - shelters also conditions for entitlement to social benefits and the relevant legislation. The empirical part defines the objectives, research methods and results, which are illustrated and described in tables, discussion and final summary. The main aim of the thesis was to find out the expectations of the mothers with children about the life in the shelter in confrontation with their real experience. The partial objectives of the thesis were to find out for what causes mothers with children come to the shelter and how their needs are met. Research questions have been identified for these objectives: For what reasons do mothers with children seek for help in the shelter? What are the expectations of mothers with children about living in the shelter? What is the real experience of mothers with children living in the shelter? Were the expectations of mothers with children about living in the shelter met? Empirical part of the thesis was processed by the method of qualitative research using semi-structured interview with opened questions. For data evaluation the method of open coding was used. The research group consisted of six women - mothers with children living in the shelter in the South Bohemian Region. Given the sensitivity of the topic the identification data of women and the shelter were not mentioned in this thesis. The research pointed out the issue of (in)availability of housing for single mothers with children in the unfavorable social situation. The most common reason for loss of housing is the break-up of the partnership, the dismissal of housing and lack of funds. The research also revealed that the main expectation of all interviewed women is to provide accommodation and stay with their children. This thesis helped to find out which services the clients seek the most and whether their needs are fulfilled.
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Possibilities of Social Work in Women with Breast Cancer
KONČICKÁ, Markéta
The theoretical part of this thesis is divided into seven chapters. In the first chapter is described the current status of the issue. The second chapter is concerned with social consequences of the disease and the third chapter is related to psychological areas of this issue. The fourth chapter is devoted to the specific stress in woman´s life caused by the breast carcinoma. The fifth chapter is focused on the disease in connection with the crisis. The sixth chapter deals with coping with problems of the disease and suffering of women. In the last chapter is defined the social work. This bachelor thesis is designed as informational material for women with breast carcinoma within professional social counseling and for social workers dealing with women with breast carcinoma.
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