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Selected microbial processes in a bioreactor
Klinková, Lucie ; doc.RNDr.Petr Zbořil, CSc. (referee) ; doc.Ing.Martin Mandl, CSc. (advisor)
This thesis focuses on the study of the influence of selected parameters on the course of microbial cultivation and evaluation of bioprocess. It is divided into two parts. The first part deals with the production of mutant forms of the protein cryptogein yeast Pichia pastoris. The theoretical part summarizes the findings of the yeast P. pastoris and its expression. It also deals with cryptogein that induces defense reactions in plants. In the experimental part was produced mutant cryptogein X24, in which the concentration of each fraction and the ability to transfer sterols. The second part of this thesis is focused on aerobic and anaerobic oxidation of elemental sulfur by Acidithiobacillus ferrooxidans. In the theoretical section, our knowledge on A. ferrooxidans, its metabolism and the importance of ATP in cell metabolism was summarized. In the experimental part, the above bioprocess was monitored using pH, biomass concentration, the rate of oxidation of elemental sulfur the cellular ATP content.
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The laboratory control of beer
Klinková, Lucie ; Pařilová, Kateřina (referee) ; Omelková, Jiřina (advisor)
This bachelor thesis is aimed on laboratory monitoring of raw materials for beer production, in-process monitoring and final product control. Theoretical part deals with variety of procedures needed to produce a quality final product. Practical part of thesis was done in a laboratory of a medium sized brewery in the Czech republic. The production of formentioned brewery is 300, 000 hectolitres of traditionally brewed beer per year. The quality control of wort and final product was done. In – process control was done to obtain the extract value and pH of wort. The clarity, color, pH, gravity, and the both apperental and real alcohol content, the degree of attenuation of apperental and real content of bitter substances was determined in final product.
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Selected microbial processes in a bioreactor
Klinková, Lucie ; doc.RNDr.Petr Zbořil, CSc. (referee) ; doc.Ing.Martin Mandl, CSc. (advisor)
This thesis focuses on the study of the influence of selected parameters on the course of microbial cultivation and evaluation of bioprocess. It is divided into two parts. The first part deals with the production of mutant forms of the protein cryptogein yeast Pichia pastoris. The theoretical part summarizes the findings of the yeast P. pastoris and its expression. It also deals with cryptogein that induces defense reactions in plants. In the experimental part was produced mutant cryptogein X24, in which the concentration of each fraction and the ability to transfer sterols. The second part of this thesis is focused on aerobic and anaerobic oxidation of elemental sulfur by Acidithiobacillus ferrooxidans. In the theoretical section, our knowledge on A. ferrooxidans, its metabolism and the importance of ATP in cell metabolism was summarized. In the experimental part, the above bioprocess was monitored using pH, biomass concentration, the rate of oxidation of elemental sulfur the cellular ATP content.
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The laboratory control of beer
Klinková, Lucie ; Pařilová, Kateřina (referee) ; Omelková, Jiřina (advisor)
This bachelor thesis is aimed on laboratory monitoring of raw materials for beer production, in-process monitoring and final product control. Theoretical part deals with variety of procedures needed to produce a quality final product. Practical part of thesis was done in a laboratory of a medium sized brewery in the Czech republic. The production of formentioned brewery is 300, 000 hectolitres of traditionally brewed beer per year. The quality control of wort and final product was done. In – process control was done to obtain the extract value and pH of wort. The clarity, color, pH, gravity, and the both apperental and real alcohol content, the degree of attenuation of apperental and real content of bitter substances was determined in final product.
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