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In vitro cytotoxicity study of selected antimycobacterial substances on human hepatocytes
Klimová, Miroslava ; Kuchařová, Monika (advisor) ; Smutná, Lucie (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biophysics and Physical Chemistry Student: Miroslava Klimová Supervisor: Mgr. Monika Kuchařová, Ph.D. Consultant: Mgr. Pavel Bárta, Ph.D. Title of diploma thesis: In vitro cytotoxicity study of selected antimycobacterial substances on human hepatocytes Clinical research is constantly progressing, new, missing drugs are being searched for, more effective substances are being synthesized, adverse effects are being minimized. The aim of this thesis was to determine the effect of ten substances (JZ195, JZ186, MH17, JZ178, MH9, MH18, JZ177, JZ170, JZ172, JZ174) in the HepG2 cell line and evaluate their cytotoxic potential. The tested substances were derived from the structure of pyrazine-2- carboxamide and synthesized within the Department of Organic and Bioorganic Chemistry at the Faculty of Pharmacy in Hradec Králové, Charles University and developed for the purpose of having promising effect on mycobacterial strains. Cytotoxicity was evaluated in vitro by CellTiter96® AQueous One Solution Cell Proliferation Assay, commercially available colorimetric method based on reduction of the tetrazolium salt to a violet-coloured formazan which directly reflects the number of viable cells. We assessed the hepatotoxic effect using the IC50...
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