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Suspension culture of Trifolium pratense L. II
Klabanová, Lenka ; Kašparová, Marie (advisor) ; Siatka, Tomáš (referee)
Lenka Klabanová Suspension Culture of Trifolium pratense L. II A principial precondition for succesful elicitation used to increase the production of secondary metabolities is finding a suitable elicitor, its concentration and the optimal period of time of the action of the elicitor on the plant culture in vitro. It was the aim of the present thesis. The effect was examined of a 6, 24, 48 and 168 hours action of the solution of aluminium trichloride (in concetrations 0,1 µmol, 1 µmol, 10 µmol, 100 µmol) on the production of flavonoids and isoflavonoids in the suspension culture of Trifolium pratense L. variety Tempus and Sprint. Culture was cultivated on a Gamborg medium with an addition of 2 mg.l-1 of 2,4-dichlorophenoxyacetic acid and 2 mg.l-1 of 6-benzylaminopurine, at the temperature of 25˚C and 16 hours light/8 hours dark period. The maximal content of flavonoids found by a photometric determination according to Pharmacopoeia Bohemica 2009 was demonstrated in the suspension culture of Trifolium pratense L. variety Tempus (0,276%) after a 168hour action of the elicitor of the 100 μmol concentration. Compared with the control culture was stimulated production of 283%. In Trifolium pratense L. variety Sprint found a maximal content (0.308%) after a 24hour action of the elicitor of the 10 μmol...
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Suspension culture of Trifolium pratense L. II
Klabanová, Lenka ; Siatka, Tomáš (referee) ; Kašparová, Marie (advisor)
Lenka Klabanová Suspension Culture of Trifolium pratense L. II A principial precondition for succesful elicitation used to increase the production of secondary metabolities is finding a suitable elicitor, its concentration and the optimal period of time of the action of the elicitor on the plant culture in vitro. It was the aim of the present thesis. The effect was examined of a 6, 24, 48 and 168 hours action of the solution of aluminium trichloride (in concetrations 0,1 µmol, 1 µmol, 10 µmol, 100 µmol) on the production of flavonoids and isoflavonoids in the suspension culture of Trifolium pratense L. variety Tempus and Sprint. Culture was cultivated on a Gamborg medium with an addition of 2 mg.l-1 of 2,4-dichlorophenoxyacetic acid and 2 mg.l-1 of 6-benzylaminopurine, at the temperature of 25˚C and 16 hours light/8 hours dark period. The maximal content of flavonoids found by a photometric determination according to Pharmacopoeia Bohemica 2009 was demonstrated in the suspension culture of Trifolium pratense L. variety Tempus (0,276%) after a 168hour action of the elicitor of the 100 μmol concentration. Compared with the control culture was stimulated production of 283%. In Trifolium pratense L. variety Sprint found a maximal content (0.308%) after a 24hour action of the elicitor of the 10 μmol...
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Foreign language classes in Kindergarten
KLABANOVÁ, Lenka
The thesis "Teaching a Foreign Language in Kindergarten" deals with foreign language education in preschool and its impact on foreign language education in primary school. The theoretical part defines the basic concepts related to preschoolers, the development specifics of preschool children, the importace of language and the development of verbal skills, and the origin of bilingualism and its risks. It also summarizes the views of experts on the early teaching of a foreign language, points out the difficulties that may occur in teaching a foreign language to preschool children, and, ultimately, focuses on appropriate methods and forms of teaching a foreign language in kindergarten. In the practical part, results of quantitative research are introduced. The research was conducted in the form of a survey and the respondents were teachers of primary schools and kindergartens. The aim was to find out how teaching of a foreign language in preschools translates into foreign language education at primary schools and if teachers perceive this activity to be effective. Furthermore, it was investigated whether there were conditions in the preprimary foreign language education permitting this initiative to be effective.
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