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Physiological interactions and stabilities of inner mitochondrial membrane complexes.
Kafková, Anežka ; Trnka, Jan (advisor) ; Mráček, Tomáš (referee) ; Vaňhara, Petr (referee)
Inner mitochondrial membrane (IMM) is composed of a phospholipid bilayer with a typically high ratio of protein complexes forming different complexes which execute different functions being reflected into the physiology of the whole organism. To maintain all these functions, as well as to have an ability to respond to various stress stimuli, the individual complexes of the respiratory chain interact, most likely to enhance the electron flow between these complexes, allowing the respiration steps from electron transfer from NADH to molecular oxygen to occur more efficiently. Other interactions between the complexes of the respiratory chain and other IMM complexes have been suggested. However, the underlying molecular details and functional relevance of such interactions are far from being elucidated. One of these many uncharacterised interactions involves the cytochrome c oxidase (COX), the terminal oxidase complex of the respiratory chain, and mitochondrial calcium uniporter complex (MCUC), the main gate to calcium entry into the mitochondrial matrix. Perhaps surprisingly, preliminary data from a systematic unbiased BioID analysis of MCUC subunits has consistently revealed an interaction between MCUC and COX subunits and assembly factors. In addition, SILAC-based quantitative proteomics also showed...
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Pathophysiological aspects of critically ill patients and its influencing with hormonal and nutritional support.
Kafková, Anežka ; Hronek, Miloslav (advisor) ; Kovařík, Miroslav (referee)
The state of critically ill patients suffering from polytrauma or sepsis is characterised by pathophysiologic processes, which cause significant changes in an organism on both a local and a systematic level. A stressful situation elicits an acute inflammatory response. This response is primarily meant to be a defence mechanism; however, this mechanism might become harmful to a certain degree. Hormones, cytokines and growth factors are included among the main control mechanisms activated during inflammatory response. A metabolic response to a stressful situation is characterized by an increased turnover of proteins, lipids, and glucose. Clinical consequences may develop as a result of these metabolic responses and include inflammation, anorexia, immobility, increased vascular permeability (which results in edema), vasodilation, tachycardia, and increased cardiac output. There are some key factors which have a negative contribution on an inflammatory response. Those factors may include malnutrition, pre-existing inflammatory activity, severe infection, obesity, ageing, genotype, and finally gender-gene effects, which may also play a role. The development of significant hormonal changes can be typified for critically ill patients. Such changes are particularly connected to catecholamines, thyroid...
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Pathophysiological aspects of critically ill patients and its influencing with hormonal and nutritional support.
Kafková, Anežka ; Hronek, Miloslav (advisor) ; Kovařík, Miroslav (referee)
The state of critically ill patients suffering from polytrauma or sepsis is characterised by pathophysiologic processes, which cause significant changes in an organism on both a local and a systematic level. A stressful situation elicits an acute inflammatory response. This response is primarily meant to be a defence mechanism; however, this mechanism might become harmful to a certain degree. Hormones, cytokines and growth factors are included among the main control mechanisms activated during inflammatory response. A metabolic response to a stressful situation is characterized by an increased turnover of proteins, lipids, and glucose. Clinical consequences may develop as a result of these metabolic responses and include inflammation, anorexia, immobility, increased vascular permeability (which results in edema), vasodilation, tachycardia, and increased cardiac output. There are some key factors which have a negative contribution on an inflammatory response. Those factors may include malnutrition, pre-existing inflammatory activity, severe infection, obesity, ageing, genotype, and finally gender-gene effects, which may also play a role. The development of significant hormonal changes can be typified for critically ill patients. Such changes are particularly connected to catecholamines, thyroid...
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