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Utilization of interfacial rheology to study of biological systems
Kachlířová, Helena ; Kalina, Michal (referee) ; Smilek, Jiří (advisor)
The aim of this bachalor thesis is to implement and optimization a relatively new method of interfacial rheology. The optimization of this method was realized on two types of interfaces, specifically liquid-liquid using immiscible systems water-chloroform and water-toluene, and water-air. The applicability of this method was tested by using real samples in form of surfactant septonex and biological system represented by protein (bovine serum albumin). The interfacial layers formed by septonex were purely viscous, where the rigidity of the layer increased slightly with concentration until reaching critical micellar concentration and then the rigidity decreased. In comparison with septonex, the interfacial film made of bovine serum albumin showed predominantly elastic behaviour.
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Interfacial rheology as the effective tool to description of interfacial behaviour of biofilms
Kachlířová, Helena ; Kráčalík, Milan (referee) ; Smilek, Jiří (advisor)
The aim of this diploma thesis is to optimize a method of interfacial rheology for testing the interfacial behaviour of biofilms on the liquid-air interface and after that use the method for studying the biofilm formation under optimal and stress conditions. For studying the biofilm formation, Kombucha was used. It is a microbial culture forming a cellulose biofilm on the interface. As the stress conditions, reduction of sucrose concentration, change of pH and change of ionic strength was used. Next, the ability of regeneration of biofilm formed on the interface was studied. The biofilm formation was occured in all cases except of increasing ionic strength. As expected, the best biofilm biofilm growth was observed under optimal condition, which means a sucrose concentration 100 g/l.
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Interfacial rheology as the effective tool to description of interfacial behaviour of biofilms
Kachlířová, Helena ; Kráčalík, Milan (referee) ; Smilek, Jiří (advisor)
The aim of this diploma thesis is to optimize a method of interfacial rheology for testing the interfacial behaviour of biofilms on the liquid-air interface and after that use the method for studying the biofilm formation under optimal and stress conditions. For studying the biofilm formation, Kombucha was used. It is a microbial culture forming a cellulose biofilm on the interface. As the stress conditions, reduction of sucrose concentration, change of pH and change of ionic strength was used. Next, the ability of regeneration of biofilm formed on the interface was studied. The biofilm formation was occured in all cases except of increasing ionic strength. As expected, the best biofilm biofilm growth was observed under optimal condition, which means a sucrose concentration 100 g/l.
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Utilization of interfacial rheology to study of biological systems
Kachlířová, Helena ; Kalina, Michal (referee) ; Smilek, Jiří (advisor)
The aim of this bachalor thesis is to implement and optimization a relatively new method of interfacial rheology. The optimization of this method was realized on two types of interfaces, specifically liquid-liquid using immiscible systems water-chloroform and water-toluene, and water-air. The applicability of this method was tested by using real samples in form of surfactant septonex and biological system represented by protein (bovine serum albumin). The interfacial layers formed by septonex were purely viscous, where the rigidity of the layer increased slightly with concentration until reaching critical micellar concentration and then the rigidity decreased. In comparison with septonex, the interfacial film made of bovine serum albumin showed predominantly elastic behaviour.
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