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Explant culture of Trifolium pratense L.
Křepelová, Jana ; Kašparová, Marie (advisor) ; Siatka, Tomáš (referee)
Jana Křepelová Explantat culture of Trifolium pratense L. A principal precondition for successful elicitation used to increase the production of secondary metabolites is, among other, finding a suitable elicitor, its concentration and the optimal period of time of action on the plant culture in vitro, which was the aim of the present thesis. The effect was examined of a 6, 24, 48 and 168 hour action of the solution of ferrous sulphate (in four concentrations) on the production of flavonoids and isoflavonoids in the suspension culture Trifolium pratense L. (variety DO-9 and variety Sprint) cultiveted on a Gamborg medium with an addition of 2 mg.l-1 of 2,4-dichlorophenoxyacetic acid and 2 mg.l-1 6- benzylaminopurine, at the temperature of 25o C and 16 hour light/ 8 hour dark period. The maximal content of flavonoids found by a photometric determination according to Pharmacopoeia Bohemica 2005 was demonstrated in the suspension culture Trifolium pratense L. (variety DO-9) (0,500 %) after 48 hour action of the elicitor of the 10 μmol concentration and in the suspension culture Trifolium pratense L. (variety Sprint) (0,415 %) also after 48 hours action 10 μmol concentration. The maximal content of isoflavonoids found by a HPLC metod was demonstrated in the suspension culture Trifolium pratense L....
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Production of phenylpropanoids in the plant explantat culture
Křepelová, Jana ; Kašparová, Marie (advisor) ; Siatka, Tomáš (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmacognosy Candidate Mgr. Jana Křepelová Consultant PharmDr. Marie Kašparová, Ph.D. Title of Thesis Production of phenylpropanoids in the plant explantat culture The aim of this Thesis was to monitor the effect on the production of phenylpropanoids after the application of the biosynthesis precursors by the plant culture in vitro. It was examined the effect of a 6, 24, 48, 72 and 168-hour exposure of the phenylalanine and cinnamic acid on the production of flavonoids and isoflavonoids in the suspension and calus culture Trifolium pratense L. (variety DO-8). The concentration of 10 mmol.l -1 of both precursors was chosen on the basis of the previous experiments. This culture was cultivated on the Gamborg medium with an addition of 2 mg.l -1 2,4-dichlorophenoxyacetic acid and 2 mg.l -1 6- benzylaminopurine, at the temperature of 25 o C and 16 hour light / 8 hour dark period. The maximal content of the flavonoids detected by the photometric determination according to Pharmacopoeia Bohemica 2009 was demonstrated in the suspension culture Trifolium pratense L. (0.262 %) after the 72-hour exposure of the phenylalanine of the 10 mmol.l -1 concentration and in the calus culture Trifolium pratense L. (0.161 %) after...
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Production of phenylpropanoids in the plant explantat culture
Křepelová, Jana ; Kašparová, Marie (advisor) ; Siatka, Tomáš (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmacognosy Candidate Mgr. Jana Křepelová Consultant PharmDr. Marie Kašparová, Ph.D. Title of Thesis Production of phenylpropanoids in the plant explantat culture The aim of this Thesis was to monitor the effect on the production of phenylpropanoids after the application of the biosynthesis precursors by the plant culture in vitro. It was examined the effect of a 6, 24, 48, 72 and 168-hour exposure of the phenylalanine and cinnamic acid on the production of flavonoids and isoflavonoids in the suspension and calus culture Trifolium pratense L. (variety DO-8). The concentration of 10 mmol.l -1 of both precursors was chosen on the basis of the previous experiments. This culture was cultivated on the Gamborg medium with an addition of 2 mg.l -1 2,4-dichlorophenoxyacetic acid and 2 mg.l -1 6- benzylaminopurine, at the temperature of 25 o C and 16 hour light / 8 hour dark period. The maximal content of the flavonoids detected by the photometric determination according to Pharmacopoeia Bohemica 2009 was demonstrated in the suspension culture Trifolium pratense L. (0.262 %) after the 72-hour exposure of the phenylalanine of the 10 mmol.l -1 concentration and in the calus culture Trifolium pratense L. (0.161 %) after...
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Explant culture of Trifolium pratense L.
Křepelová, Jana ; Siatka, Tomáš (referee) ; Kašparová, Marie (advisor)
Jana Křepelová Explantat culture of Trifolium pratense L. A principal precondition for successful elicitation used to increase the production of secondary metabolites is, among other, finding a suitable elicitor, its concentration and the optimal period of time of action on the plant culture in vitro, which was the aim of the present thesis. The effect was examined of a 6, 24, 48 and 168 hour action of the solution of ferrous sulphate (in four concentrations) on the production of flavonoids and isoflavonoids in the suspension culture Trifolium pratense L. (variety DO-9 and variety Sprint) cultiveted on a Gamborg medium with an addition of 2 mg.l-1 of 2,4-dichlorophenoxyacetic acid and 2 mg.l-1 6- benzylaminopurine, at the temperature of 25o C and 16 hour light/ 8 hour dark period. The maximal content of flavonoids found by a photometric determination according to Pharmacopoeia Bohemica 2005 was demonstrated in the suspension culture Trifolium pratense L. (variety DO-9) (0,500 %) after 48 hour action of the elicitor of the 10 μmol concentration and in the suspension culture Trifolium pratense L. (variety Sprint) (0,415 %) also after 48 hours action 10 μmol concentration. The maximal content of isoflavonoids found by a HPLC metod was demonstrated in the suspension culture Trifolium pratense L....
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