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Vliv polynenasycených mastných kyselin na zastoupení mastných kyselin ve tkáních a hladinu cholesterolu u potkanů
Kácalová, Tereza
The aim of this work was demonstrate positive effect of n-3 polyunsaturated fatty acids EPA and DHA on the concentration of plasma triglycerides and cholesterol. According to the hypothesis, these polyunsaturated fatty acids are capable of reducing plasma cho-lesterol in mechanism, where is increased gene expression Insig-1 and simultaneously reduces the expression of genes encoding an enzyme for cholesterol synthesis (3-hydroxy-3-methylglutaryl-CoA reductase, a gene for HMG-CoA-R) and the gene for the LDL receptor, which is responsible for the entry of cholesterol from the plasma into the cells (low density lipoprotein receptor, the density, the LDL-R gene). LDL-R.The experiment was conducted on a model animal (Rattus norvegicus) fed with a standard feed mixture with the addition of 3% of salmon oil, fish oil 6% and 6% of oil from algae of the genus Schizochytrium. The control group was fed a feed mixture with the addition of 6% safflower oil, food with the addition of 3% palm oil was used as a negative control. Each group consisted of 10 animals. From the blood samples was determined levels of total cholesterol, HDL-cholesterol, LDL-cholesterol and triglycerides. The fatty acid content was determined in muscle, liver and adipose tissue. Food enriched with eicosapentaenoic acid and docosahexaenoic acid led to a reduction in total cholesterol and LDL-cholesterol levels. It was shown (P <0.05) after the addition of fish oil, salmon oil and algae of the genus Schizochytrium oil to feed mixtures of experimental rats, a decrease in total cholesterol (27%, 21%, 27% respectively) relative to controls. Our attempt partially confirmed the hypothesis. At the same time, we found that plasma lipid metabolism affects different mechanism than we thought.
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