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In-situ analysis of photosynthetic pigments in bacterial cells
Janošec, Juraj ; Vodička, Juraj (referee) ; Slaninová, Eva (advisor)
This bachelor's thesis deals with the design and characterization of an appropriate combination of methods for the analysis of photosynthetic pigments in a model organism that produces such pigments. Specifically, the microorganism Rhodospirillum rubrum was investigated, which, in addition to photosynthetic pigments, also produces significant products such as potential plastic substitutes called polyhydroxyalkanoates. Structural and quantitative composition of the pigments was analyzed under different cultivation conditions with respect to light conditions. The methods used included spectroscopic techniques, particularly UV-VIS spectroscopy. Specifically, single-beam and double-beam UV-VIS spectroscopy in transmission and diffuse transmission modes, as well as fluorescence spectroscopy, were employed. These methods confirmed the presence of bacteriochlorophyll and several carotenoids, with the highest representation being of spirilloxanthin.
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