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Vliv vitamínů rozpustných v tucích na reprodukci mléčného skotu
Jandová, Romana
Abstract Fertility is an important factor in dairy cattle breeding. Temperature stress, lack of food, insufficient amount of vitamins and minerals in the ration or diseases can have a negative effect on it. As stated in this work, fertility is mainly influenced by the content of fat-soluble vitamins, especially vitamin E. Many authors describe its beneficial effect on spermatogenesis in males, while in females it helps maintain the fetus or ensures a suitable environment in the uterus. Vitamin E deficiency causes delayed sexual maturity and low libido of bulls. In females, keratinization of the epithelium or absorption of a non-prosperous fetus. I work as an insemination technician. In my work, I described the influence of fat-soluble vitamins on fertility. At the same, in the chapter "Personal experience" I described the effect of the addition of a preparation with protected fats and the addition of a vitamin supplement on the fertility of dairy cows. From the results of my monitoring, it follows that these preparations had a beneficial effect on fertility. Thanks to the protected fats, there was also a better absorption of fat-soluble vitamins (A, D, E) and thus a lower incidence of cysts and a higher percentage of pregnant cows after insemination.
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Study of interleukin 37 and its role in rheumatoid arthritis
Jandová, Romana ; Šenolt, Ladislav (advisor) ; Krulová, Magdaléna (referee)
Dysregulation between pro- and anti-inflammatory cytokines activity in rheumatoid arthritis (RA) contributes to immune dysregulation, chronic inflammation and subsequent joint destruction. Interleukin-37 (IL-37) has been described as an anti-inflammatory cytokine in several autoimmune diseases. The main aim of this work was to determine the levels of IL-37 in serum and synovial fluid (SF) of RA patients and to compare them with the levels in patients with osteoarthritis (OA) and further explore the association of IL-37 with disease activity and other clinical parameters. Subsequent goal was to study its anti-inflammatory function on RA synovial fibroblasts and describe other cells types of synovial tissue contributing to its production. IL-37 levels were detected using enzyme-linked immunosorbent assay (ELISA). Synovial fibroblasts were stimulated by lipopolysaccharide (LPS) and recombinant IL-37 (rIL-37). The levels of studied genes were detected by PCR. Synovial tissues and immune cells were visualized by immunohistochemical and by immunofluorescence staining. We found increased levels of IL-37 in SF of patients with RA in comparison to OA patients. There was a significant correlation between serum and SF levels of IL-37. RA as well as OA patients showed increased levels of IL-37 in serum than in...
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Study of interleukin 37 and its role in rheumatoid arthritis
Jandová, Romana ; Šenolt, Ladislav (advisor) ; Krulová, Magdaléna (referee)
Dysregulation between pro- and anti-inflammatory cytokines activity in rheumatoid arthritis (RA) contributes to immune dysregulation, chronic inflammation and subsequent joint destruction. Interleukin-37 (IL-37) has been described as an anti-inflammatory cytokine in several autoimmune diseases. The main aim of this work was to determine the levels of IL-37 in serum and synovial fluid (SF) of RA patients and to compare them with the levels in patients with osteoarthritis (OA) and further explore the association of IL-37 with disease activity and other clinical parameters. Subsequent goal was to study its anti-inflammatory function on RA synovial fibroblasts and describe other cells types of synovial tissue contributing to its production. IL-37 levels were detected using enzyme-linked immunosorbent assay (ELISA). Synovial fibroblasts were stimulated by lipopolysaccharide (LPS) and recombinant IL-37 (rIL-37). The levels of studied genes were detected by PCR. Synovial tissues and immune cells were visualized by immunohistochemical and by immunofluorescence staining. We found increased levels of IL-37 in SF of patients with RA in comparison to OA patients. There was a significant correlation between serum and SF levels of IL-37. RA as well as OA patients showed increased levels of IL-37 in serum than in...
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Laboratory diagnosis of Clostridium difficile
JANDOVÁ, Romana
Clostridium difficile toxin with production is the most common cause of nosocomial enteric infections. It causes inflammatory bowel disease called Clostridium difficile infection (CDI) of varying severity - from trivial diarrhea to life-threatening conditions such as paralytic ileus and toxic megacolon. C.difficile still escapes the attention of the general public and is in the background of other bacteria, such as MRSA. Clostridium difficile is a strictly anaerobic bacterium. It is a gram-positive rod forming an oval subterminal spores. C. difficile can produce two types of toxins - and toxin A (enterotoxin) and toxin B (a cytotoxin). Due to the toxin causes damage of the intestinal epithelium and deeper layers of the bowel wall. Into inflammatory ulceration that cover of the pseudomembrane. Some strains produce more binary toxin, whose exact function is not underwood yet. It is assumed that potentiates the effect of toxins A and B to increase their concentration. To demonstrate the CDI must remove the stool sample into a sterile tube. In the bacteriology workplace in Czech Budweis Hospital is being made direct conclusiveness of antigen and toxin by immuncgromatography method. It is a membrane-enzyme immunoanalysis for the detection of antigen - glutamate dehydrogenase (GDH) and toxin A / B. Negative result excludes GDH with high reliability clostridial infection. For confirmed CDI is considered proof toxigenic strain of C. difficile. This is evident from the result of either immunochromatography which is demonstrated both the antigen and the toxin, or identity of the gene for toxin B by PCR. If you find out in direct detection only positive antigen, the sample is sent for PCR testing to the Laboratory of Molecular Biology and Genetics. Regardless of the outcome of the immunochromatography, the microscopic specimen stained by Gram are being produce from reces. In the case of proven C. difficile toxin is carried anaerobic culture test that takes two days. For identification of accrued bacteria is used latex agglutination. This is a method in which IgG antibodies bound to latex particles specifically bind to the antigens of the cell wall. To identify the bacteria can be also used the VITEK - MS machine that uses a laser ionization method in the presence of the matrix, followed by mass spectrometry. In case of a positive culture findings Clostridium difficile is being from grown culture set sensitivity to antibiotics by E-test and disk diffusion methods For the year 2011 the Department of Bacteriology examined 291 samples of feces. 13,4 % of these samples had positive antigen and toxin in an imunochromatographyc examination; 15,1 % had a positive antigen and negative toxin and 71,5 % were negative. 74 samples was positive in C. difficile toxin. These samples were subsequently conducted to culture examination - 62,2 % were positive. Most positive samples were sent from the infectious department. It has been examined 53,6 % of women and 46.4 % of mens. Percentage of toxigenic strains of both sexes were virtually identical - 25 %. All strains were susceptible to vancomycin and only one strain was resistant to metronidazole.
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Research of Value Orientation in Students of Secondary School and High School, Purpose the Intervention Program.
JANDOVÁ, Romana
Thesis deals with the value orientation of students in secondary schools and secondary vocational schools. The aim is to map their value system and then based on the results of research to design an intervention program to promote internalization of values desired. The theoretical part includes a systematic search of the available literature values and their hierarchy, the age period of adolescence, the ways and possibilities of educating students given age period and the role of schools in shaping values and their internalization. The main method of the practical part of the work is own questionnaire assessing the value orientation of students in selected secondary schools and secondary vocational schools. On the basis of the results was designed educational intervention program. Results are shown for better clarity in written and graphical form. The program is described in detail. There are given a specific samples teaching unit on the topic for the development of desired values of the students. The program could become educational material for teachers of health education and other relatives.
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