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Monitoring of expression changes of selected markers in glucose-induced endothelial dysfunction in vitro.
Hudecová, Alexandra ; Nachtigal, Petr (advisor) ; Rathouská, Jana (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Title of Diploma Thesis: Monitoring the change in expression of selected indicators in the glucose induced endothelial dysfunction in vitro. Author: Alexandra Hudecová Supervisor of Diploma Thesis: prof. PharmDr. Petr Nachtigal, Ph.D. Background: The aim of this diploma thesis was to determine, whether the new monoclonal antibody carotuximab affects the expression of endoglin and other biomarkers of endothelial dysfunction (eNOS, ICAM-1, VCAM-1, E-selectin) in human aortic endothelial cells exposed to high glucose. Methods: Human aortic endothelial cells (HAEC) were exposed to high glucose levels (45 mmol) for 72 hours and carotuximab (300 µg/ml) for 12 hours. We measured the gene expression of endoglin, eNOS, ICAM-1, VCAM-1, E-selectin by real-time PCR. We measured endoglin and ICAM-1 protein levels by flow cytometry. Results: Gene expression of endoglin and all biomarkers of endothelial dysfunction was significantly increased after high glucose. After the addition of carotuximab to high glucose, we observed a significant increase in gene expression of eNOS, while no significant difference was measured in endoglin or other markers of endothelial dysfunction. Protein levels of endoglin were...
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Monitoring of expression changes of selected markers in glucose-induced endothelial dysfunction in vitro.
Hudecová, Alexandra ; Nachtigal, Petr (advisor) ; Rathouská, Jana (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Title of Diploma Thesis: Monitoring the change in expression of selected indicators in the glucose induced endothelial dysfunction in vitro. Author: Alexandra Hudecová Supervisor of Diploma Thesis: prof. PharmDr. Petr Nachtigal, Ph.D. Background: The aim of this diploma thesis was to determine, whether the new monoclonal antibody carotuximab affects the expression of endoglin and other biomarkers of endothelial dysfunction (eNOS, ICAM-1, VCAM-1, E-selectin) in human aortic endothelial cells exposed to high glucose. Methods: Human aortic endothelial cells (HAEC) were exposed to high glucose levels (45 mmol) for 72 hours and carotuximab (300 µg/ml) for 12 hours. We measured the gene expression of endoglin, eNOS, ICAM-1, VCAM-1, E-selectin by real-time PCR. We measured endoglin and ICAM-1 protein levels by flow cytometry. Results: Gene expression of endoglin and all biomarkers of endothelial dysfunction was significantly increased after high glucose. After the addition of carotuximab to high glucose, we observed a significant increase in gene expression of eNOS, while no significant difference was measured in endoglin or other markers of endothelial dysfunction. Protein levels of endoglin were...
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