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	Distribution and design of tetracycline resistance determinants in the environment Hucková, Tereza ; Lichá, Irena (advisor) ; Beranová, Jana (referee) Detailed record
	Genetic characterization of tetracycline resistance in selected soil isolates of bacteria. Hucková, Tereza ; Lichá, Irena (advisor) ; Janata, Jiří (referee) GENETIC CHARACTERIZATION OF TETRACYCLINE RESISTANCE IN SELECTED SOIL ISOLATES OF BACTERIA Abstract Antibiotic resistance is becoming increasingly widespread among bacterial organisms. To respond to the situation it is necessary to understand in detail all the mechanisms of resistance as well as expansion possibilities of resistance genes in the environment. In this study we attempted to identify tetracycline resistance determinants in selected soil gram-positive and gram-negative isolates. The isolates originate from unfertilized soil and from soil fertilized with tetracycline-contaminated manure. We tested the soil isolates for the presence of twenty three selected tetracycline resistance determinants and presence of tetracycline resistance determinants in DNA libraries. We identified one of the tetracycline resistance determinants tet(K) in Staphylococcus sp. A DNA fragment was amplified with primers for tet(M) determinant in Arthrobacter sp., but its presence was not confirmed by the sequence analysis. None of the tested tetracycline resistance determinants were present in the genera Chryseobacterium and Stenotrophomonas. However, we managed to prove the ongoing horizontal gene transfer between the genus Stenotrophomonas and the genus Chryseobacterium. The transferred sequences encoded hybrid protein and... Detailed record
	Distribution and design of tetracycline resistance determinants in the environment Hucková, Tereza ; Beranová, Jana (referee) ; Lichá, Irena (advisor) Detailed record

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