|
| |
|
|
Chemosenzitivity testing of human ovarian cancer cells and its in vitro model, human ovarian cancer cell line A2780
Caltová, Kateřina ; Červinka, Miroslav (advisor) ; Vaňásek, Jaroslav (referee) ; Hofmanová, Jiřina (referee)
Ovarian cancer is the fifth most common cancer and the most frequent cause of the death among gynaecological cancers in population of Czech women. The latest data from the National oncological register of Czech Republic show the incidence 20,64 and mortality 12,58 in 100 000 women (2010). A serious problem is represented by a late diagnosis of cancer, when the disease is diagnosed in the late stadium and finally this causes high mortality from this kind of cancer. Most complicated factors include high heterogenity of the disease, frequent apperance of resistance to cytostatics and low individualization of chemotherapy. In cooperation with a Clinic of gynaecology and obstetrics, Faculty hospital in Hradec Kralove, we have tested solid tumors and ascites samples of patients with ovarian cancer diagnosis to predict answers to chemotherapy within in vitro conditions. A panel of six cytostatics (cisplatin, paclitaxel, carboplatin, gemcitabine, topotecan, etoposide), which are also used in primary chemotherapy of ovarian cancer, has been tested. We have also used a model system, a human ovarian cancer cell line A2780, co compare the reactivity to tested cytostatics. Our results show the highest sensitivity of cells isolated from clinical samples of solid tumors and ascitic fluid to topotecan and...
|
|
|
Účinky seleničitanu sodného na buňky lidského kolorektálního karcinomu s odlišným p53 genotypem
Králová, Věra ; Rudolf, Emil (advisor) ; Skálová, Lenka (referee) ; Hofmanová, Jiřina (referee)
Effects of sodium selenite in human colon cancer cells with different p53 status Colorectal carcinoma (CRC) is one of the leading causes of cancer - associated mortality. Great effort is spent on the investigation of prevention possibilities by various types of environmental factors. Several epidemiological studies and clinical studies have shown an inverse association between selenium intake and the risk of different types cancer including CRC. Also in animal models and cell lines in vitro selenium compounds have shown ability to inhibit proliferation and induce cell death. The aim of this study was to assess the antiproliferative effects of sodium selenite in colorectal cancer cells in vitro, particularly in two model colorectal cancer cell lines HCT 116 differing in their p53 status. We have shown that sodium selenite in concentration range of 2.5 µM-10 µM inhibited proliferation and induced cell death in HCT 116 cells in a time- and concentration- dependent manner. Sodium selenite blocked the progression through the cell cycle in the S/G2-M phases, induced superoxide production in mitochondria, DNA damage and expression of p53 protein and activated cell death with involvement of caspases. Sodium selenite also increased the expression of autophagy markers. The HCT 116-p53KO cells, lacking...
|
|
| |
|
| |
|
| |
|
|
Polyunsaturated fatty acids (PUFAs) enhance the sensitivity of HT-29 human colon adenocarcinoma cell line to apoptotic effects of butyrate, anti-Fas and TNF-alpha
Vaculová, Alena ; Hofmanová, Jiřina ; Kovaříková, Martina ; Souček, Karel ; Šimek, V. ; Zadák, Z. ; Kozubík, Alois
Práce vede k závěrům, že vysoce nenasycené mastné kyseliny (pufas) zvyšují citlivost buněčné línie lidského adenokarcinomu kolonu ht-29 k apoptickým účinkům butyrátu, anti-fas a tnf-alpha
|
|
| |
|
| |
|
| |
guest ::
RSS feed.