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Laboratory evaluation of activity of traditional Bolivian medicinal plants
 Hladová, Alena ; Klouček, Pavel (advisor) ; Přemysl, Přemysl (referee)
Traditional medicine of indigenous cultures is based on the medicinal effects of local plants. Those people have been taught to use these plants throughout history and they employ them as their primary health care. This is supported by the fact that the natives do not have the financial resources to pay for medical care and at the same time it is also accompanied by poor availability of hospitals in areas where these cultures are located. This thesis covers laboratory verification of antimicrobial and antioxidant activity of Amazonian plants from south west Bolivia. This paper also summarizes the economic and political situation of the country together with the development of traditional medicine in the world and with the potential use of this medicine in pharmacy. Part of the work is devoted to the issue of ever-increasing antimicrobial resistance and the possible effects of free radicals in the human body. The experiment was performed by testing nine samples of imported dried plants in laboratory. To determine the antimicrobial properties a microdilution method was used and the antioxidant activity was verified with the ORAC method which is based on the elimination of free radicals. The microdilution method confirmed 80% of the MIC in the initial concentrations tested (1,024 mg/ml) in Satureja ovata plants against Staphylococcus aureus ATCC 33591 bacteria and Streptococcus pyogenes bacteria and in the plant Baccharis genistelloides against S. pyrogenes bacteria. The other samples have failed to provide the value of the minimum inhibitory concentrations. This can be attributed to several factors which are described in this thesis. In contrast the positive effect free radicals was confirmed in all samples. The highest efficacy showed Satureja ovata (1373,2 ug TE/mg extract). Writing this thesis was accompanied by a lack of information about the tested plants, both in terms of their botanical description and in terms of comparing the laboratory results obtained with the results of specialized studies. Therefore the results of the experiment were studied in comparison to traditional use of the tested plants that have been provided by Bolivian natives or their effects have been analyzed from the literature.
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2 Hladová, Anežka
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