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Compact City - or what new is able to yet be done in Brno among railway stations
Hill, Petr ; Palacký, Jiří (referee) ; Havliš, Karel (advisor)
Diploma project seeks for new visions in urban solutions of area south of Brno main railway station. This place could become an interesting space for new spacial creativity in connection with the project of new railway station. The work looks for alternatives to officially presented projects with orderly and straight street layouts to create pleasing and livable public space, which would become not only the tour from station to the city, but the city itself. Six meters of height difference is used to separate road and pedestrian traffic and offers an undisturbed walking movement through the elevated pedestrian streets, which are not so deep as they would be when on the ground level, so their width could be downsized to a pleasant minumum, whereas all necesary functions (like parking and supplies areas) také place below it. Geometrical concept of two shifted hexagonal grids delivers a practical large blocks on the ground level for parking and divides the area into separate projects. The walking streets` grid fractionates big portions into more subtle building block layout so comfortable for pedestrian movement. Project also deals with development phases of area to protect it from nonreversible tenous development at the beginning. The structure works as a lively city in the very beginning of the development.
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Compact City - or what new is able to yet be done in Brno among railway stations
Hill, Petr ; Palacký, Jiří (referee) ; Havliš, Karel (advisor)
Diploma project seeks for new visions in urban solutions of area south of Brno main railway station. This place could become an interesting space for new spacial creativity in connection with the project of new railway station. The work looks for alternatives to officially presented projects with orderly and straight street layouts to create pleasing and livable public space, which would become not only the tour from station to the city, but the city itself. Six meters of height difference is used to separate road and pedestrian traffic and offers an undisturbed walking movement through the elevated pedestrian streets, which are not so deep as they would be when on the ground level, so their width could be downsized to a pleasant minumum, whereas all necesary functions (like parking and supplies areas) také place below it. Geometrical concept of two shifted hexagonal grids delivers a practical large blocks on the ground level for parking and divides the area into separate projects. The walking streets` grid fractionates big portions into more subtle building block layout so comfortable for pedestrian movement. Project also deals with development phases of area to protect it from nonreversible tenous development at the beginning. The structure works as a lively city in the very beginning of the development.
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Potravní zdroje bezobratlých v Ardovské jeskyni a v jeskyni Domica - předběžné výsledky
Nováková, Alena ; Elhottová, Dana ; Krištůfek, Václav ; Lukešová, Alena ; Hill, P. ; Kováč, L. ; Mock, A. ; Luptáčik, P.
The chemistry (organic matter content and pH) of different substrates from the Ardovská and Domica Caves in the Slovak Karst National Park (Slovakia) and the composition of their microbial communities were studied. Differences in organic matter content and microbial communities were found between the substrates from the Domica and Ardovská Caves. A broad spectrum of photoautotrophic microorganisms (algae and cyanobacteria) was found in different cave microhabitats (cave sediment, rotten wood, earthworms casts and isopods faeces), but no algae were detected in the fresh bat droppings and in the intestinal tract of the isopod .i.M. graniger./i.. Microfungi and bacterial colonies play the primary role in transforming bat guano into a food source for saprophagous and microphytophagous invertebrates.
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Použití specifických aktinomycetových PCR primerů k charakterizaci bakteriálních společenstev exkrementů žížal
Hill, P. ; Krištůfek, Václav ; Feijoo, A. M. ; Gallego, G.
Tropical geophageous earthworms stimulate soil bacterial activity in soil that they ingest; it is uncertain if they affect the whole bacterial population or only subgroups. We sampled .i.Martiodrillus heterostichon, Polypheretima elongata./i. and bulk soil from a Bamboo plantation near Cali, Colombia. Pots of 1500 g of an adjacent tobacco field soil were brought to field capacity and two worms of each species placed in each pot. .i.Martiodrillus./i. gut contents and wormcasts from the soil surface and within tunnels were sampled after three months. The soil was maintained at field capacity for a further five months during which the worms continued to produce casts. Worm cast Amplified ribosomal DNA restriction analysis ARDRA (using Tag I) with actinomycete specific primers gave two strong bands at approximately 750 and 370 bp. ARDRA patterns from the three bamboo grove and four field soils had several additional bands notably at 140 and 126 bp.
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Charakteristika různorodých půd a sedimentů užitím specifických aktinomycetových r-DNA PCR primerů
Hill, P. ; Krištůfek, Václav ; Caballero, S. ; Kroetsch, D. J. ; Rauch, Ota
Most molecular biological studies of the soil characterise a few soils or sediments under a range of treatments or over a limited time. It is difficult to compare results between studies due to variations in DNA extraction method and PCR conditions. High diversity of the soil bacterial population means that it is impossible to characterise many soils through cloning and sequencing and that finger prints are often blurred and difficult to interpret. We compare a broad range of soils and zoo/anthropogenic sediments with actinomycete specific primers. ARDRA and cloning showed that these primers amplified mainly verrucomicrobia from tropical, temperate and sub-arctic soils and actinomycetes from biologically active and urban street sediments. We found two new groups that were broadly distributed in soils: a new actinomycete family and the AD-3 candidate division that was found in acid tropical and sub-arctic soils
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