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Effect of anthelmintics on activity of selected biotranformation enzymes in plants
Hanulíková, Markéta ; Raisová Stuchlíková, Lucie (advisor) ; Jansová, Hana (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Markéta Hanulíková Supervisor: RNDr. Lucie Raisová Stuchlíková, Ph.D. Consultant: RNDr. Radka Podlipná, Ph.D. Title of diploma thesis: Effect of anthelminthics on activity of selected biotransformation enzymes Anthelmintics belong between the drugs widly used in veterinary medicine. In the environment, these drugs represent a significant source of pollution with a negative impact on micro-organism and entire ecosystems. The fate of anthelmintics and their effect on plants has not yet been studied sufficiently. The aim of this work was to investigate the effect of two frequently used anthelmintics, fenbendazole (FEN) and ivermectin (IVM) on antioxidant enzymes peroxidase (POX), catalase (CAT), ascorbate peroxidase (APX), glutathione reductase (GR), glutathione-S-transferase (GST) and superoxide dismutase (SOD) in model plant Arabidospsis thaliana. In this study was used in vitro cell suspension of Arabidospsis thaliana. Enzyme activity was measured spectrophotometrically in samples taken after 8, 24 and 72 hours. At samples exposed to effect of FEN the activity of SOD enzyme increased, on the other hand the activity of GST enzyme decreased. The presence of IVM has an inhibitory effect on GR, GST...
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Effect of anthelmintics on activity of selected biotranformation enzymes in plants
Hanulíková, Markéta ; Raisová Stuchlíková, Lucie (advisor) ; Jansová, Hana (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Markéta Hanulíková Supervisor: RNDr. Lucie Raisová Stuchlíková, Ph.D. Consultant: RNDr. Radka Podlipná, Ph.D. Title of diploma thesis: Effect of anthelminthics on activity of selected biotransformation enzymes Anthelmintics belong between the drugs widly used in veterinary medicine. In the environment, these drugs represent a significant source of pollution with a negative impact on micro-organism and entire ecosystems. The fate of anthelmintics and their effect on plants has not yet been studied sufficiently. The aim of this work was to investigate the effect of two frequently used anthelmintics, fenbendazole (FEN) and ivermectin (IVM) on antioxidant enzymes peroxidase (POX), catalase (CAT), ascorbate peroxidase (APX), glutathione reductase (GR), glutathione-S-transferase (GST) and superoxide dismutase (SOD) in model plant Arabidospsis thaliana. In this study was used in vitro cell suspension of Arabidospsis thaliana. Enzyme activity was measured spectrophotometrically in samples taken after 8, 24 and 72 hours. At samples exposed to effect of FEN the activity of SOD enzyme increased, on the other hand the activity of GST enzyme decreased. The presence of IVM has an inhibitory effect on GR, GST...
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Effect of anthelmintics on activity of selected biotranformation enzymes in plants
Hanulíková, Markéta ; Raisová Stuchlíková, Lucie (advisor) ; Jansová, Hana (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Markéta Hanulíková Supervisor: RNDr. Lucie Raisová Stuchlíková, Ph.D. Consultant: RNDr. Radka Podlipná, Ph.D. Title of diploma thesis: Effect of anthelminthics on activity of selected biotransformation enzymes Anthelmintics belong between the drugs widly used in veterinary medicine. In the environment, these drugs represent a significant source of pollution with a negative impact on micro-organism and entire ecosystems. The fate of anthelmintics and their effect on plants has not yet been studied sufficiently. The aim of this work was to investigate the effect of two frequently used anthelmintics, fenbendazole (FEN) and ivermectin (IVM) on antioxidant enzymes peroxidase (POX), catalase (CAT), ascorbate peroxidase (APX), glutathione reductase (GR), glutathione-S-transferase (GST) and superoxide dismutase (SOD) in model plant Arabidospsis thaliana. In this study was used in vitro cell suspension of Arabidospsis thaliana. Enzyme activity was measured spectrophotometrically in samples taken after 8, 24 and 72 hours. At samples exposed to effect of FEN the activity of SOD enzyme increased, on the other hand the activity of GST enzyme decreased. The presence of IVM has an inhibitory effect on GR, GST...
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