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Rifampicin loaded polymeric nanoparticles
Hamplová, Karolína ; Šnejdrová, Eva (advisor) ; Dittrich, Milan (referee)
Charles University Faculty of Pharmacy in Hradec Kralove Department of Pharmaceutical Technology Author: Karolína Hamplová Title of thesis: Polymer nanoparticles with rifampicin Supervisor: PharmDr. Eva Šnejdrová, Ph.D. The aim of the work was to formulate nanoparticles (NPs) with rifampicin by nanoprecipitation using polyesters of lactic acid and glycolic acid of linear or branched architecture. NPs were characterized by size, polydispersity, zeta potential, and scanning electron microscopy (SEM). Encapsulation efficacy (EE), loading capacity (LC), and dissolution profile of rifampicin were determined. NPs were stabilized by lyophilization, the effectiveness of cryoprotectants was tested and NPs were characterized after reconstitution. Although the size of the NP can be modified by a number of formulation factors, the concentration of the polymer in the internal phase and the molar weight of the polymer play a crucial role. Surprisingly promising for the formulation of nanoparticles with rifampicin by the nanoprecipitation method was the polymer PLGA 70:30. A 0.5% poloxamer solution was the most effective for steric stabilization of nanoparticles under given formulation conditions, and a 0.01% solution of didodecyldimethylammonium bromide was the most effective for electrostatic stabilization. SEM...
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Rifampicin loaded polymeric nanoparticles
Hamplová, Karolína ; Šnejdrová, Eva (advisor) ; Dittrich, Milan (referee)
Charles University Faculty of Pharmacy in Hradec Kralove Department of Pharmaceutical Technology Author: Karolína Hamplová Title of thesis: Polymer nanoparticles with rifampicin Supervisor: PharmDr. Eva Šnejdrová, Ph.D. The aim of the work was to formulate nanoparticles (NPs) with rifampicin by nanoprecipitation using polyesters of lactic acid and glycolic acid of linear or branched architecture. NPs were characterized by size, polydispersity, zeta potential, and scanning electron microscopy (SEM). Encapsulation efficacy (EE), loading capacity (LC), and dissolution profile of rifampicin were determined. NPs were stabilized by lyophilization, the effectiveness of cryoprotectants was tested and NPs were characterized after reconstitution. Although the size of the NP can be modified by a number of formulation factors, the concentration of the polymer in the internal phase and the molar weight of the polymer play a crucial role. Surprisingly promising for the formulation of nanoparticles with rifampicin by the nanoprecipitation method was the polymer PLGA 70:30. A 0.5% poloxamer solution was the most effective for steric stabilization of nanoparticles under given formulation conditions, and a 0.01% solution of didodecyldimethylammonium bromide was the most effective for electrostatic stabilization. SEM...
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