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Development and validation of HPLC method for determination of impurity 2-ethylpyridine in topical gel containing dimetindene maleate
Hudcová, Petra ; Hájková, Renata (advisor) ; Matysová, Ludmila (referee)
Title of graduation theses: Development and validation of HPLC method for determination of impurity 2-ethylpyridine in topical gel containing dimetindene maleate Petra Hudcová Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Analytical Chemistry A High Performance Liquid Chromatography method for the simultaneous determination of 2-ethylpyridine and dimetindene maleate in Fenistil® gel was developed and validated. Analytical column Supelco Discovery® Cyano (100 x 4 mm; 5 μm) and mobile phase composed of acetonitrile:ammonium secondary phosphate buffer 50 mM, pH 5.5, with addition of 0.5 ml of triethylamine in 100 ml buffer, 40:60 (v/v) were used for the chromatography. The flow-rate was 0.7 mL/min and the UV detection was accomplished at 258 nm. Diltiazem hydrochloride was used as internal standard. Substances were eluted in the following order: 2-ethylpyridine, diltiazem hydrochloride, dimetindene maleate. Validation parameters precision, accuracy, linearity, robustness, selectivity, limit of detection and limit of quantication were calculated. The system suitability test data (number of theoretical plates, asymmetry, resolution and repeatability of area and retention time) were tested.
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Monitoring the impact of exposure to electromagnetic fields in yeast colonies
Hájková, Renata ; Vorlíček, Jaroslav (advisor) ; Cifra, Michal (referee)
Identifikační záznam: HÁJKOVÁ, Renata. Sledování vlivu expozice elektromagnetickým polem na kolonii kvasinek [Monitoring the impact of exposure to electromagnetic fields in yeast colonies]. Praha, 2011, rok vydání. 74s., 2. příl. Diplomová práce (Mgr.). Univerzita Karlova v Praze, 1. Lékařská fakulta, Fakulta elektrotechnická, Katedra elektromagnetického pole, ČVUT v Praze. Vedoucí závěrečné práce Vorlíček, Jaroslav, Ing. Abstract: Current development of mobile telecommunication device increases interest of public to the question of influence of electromagnetic field to the living organisms. Results of this work contribute to better knowledge of interaction between electromagnetic field and cell structures. Beginning of the thesis is review of morphology and physiology of the yeast cell that was used in this work as a tested organism. Second chapter describe the basic facts about electromagnetic fiend and wireless energy transfer. Goal of the practical part of this master thesis is describe influence of electromagnetic field on population of yeast during their proliferation. We constructed special box connected with wireless energy generator that was source of exactly defined electromagnetic field. Cells of Saccharomyces cerevisiae were diluted in fluid medium YPD and the proliferation of the culture was...
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Development and validation of HPLC method for determination of impurity 2-ethylpyridine in topical gel containing dimetindene maleate
Hudcová, Petra ; Hájková, Renata (advisor) ; Matysová, Ludmila (referee)
Title of graduation theses: Development and validation of HPLC method for determination of impurity 2-ethylpyridine in topical gel containing dimetindene maleate Petra Hudcová Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Analytical Chemistry A High Performance Liquid Chromatography method for the simultaneous determination of 2-ethylpyridine and dimetindene maleate in Fenistil® gel was developed and validated. Analytical column Supelco Discovery® Cyano (100 x 4 mm; 5 μm) and mobile phase composed of acetonitrile:ammonium secondary phosphate buffer 50 mM, pH 5.5, with addition of 0.5 ml of triethylamine in 100 ml buffer, 40:60 (v/v) were used for the chromatography. The flow-rate was 0.7 mL/min and the UV detection was accomplished at 258 nm. Diltiazem hydrochloride was used as internal standard. Substances were eluted in the following order: 2-ethylpyridine, diltiazem hydrochloride, dimetindene maleate. Validation parameters precision, accuracy, linearity, robustness, selectivity, limit of detection and limit of quantication were calculated. The system suitability test data (number of theoretical plates, asymmetry, resolution and repeatability of area and retention time) were tested.
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