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Conservation genetics of the grey wolf in Central Europe
Valentová, Kamila Anna ; Hulva, Pavel (advisor) ; Galov, Ana (referee)
Conservation genetics of the grey wolf in Czech Republic and adjacent regions is studied in the present thesis. Analyses of twenty-one microsatellite loci, one sex-determining amelogenin gene and mitochodrial control region were used to verify species determination, identify individuals and estimate relationships between them, analyse population structure and estimate demographic trends based on samples collected between 2014 and 2021. Genetic detection of red fox and dog samples incorrectly assigned to wolves illustrates the hurdles of field monitoring of grey wolf. Direct evidence for the occurrence of F1 hybrids was not found. Wolves from Bohemia showed lower values of allelic richness in comparison to the ones from Western Carpathians, probably as a consequence of recent expansion. Geographic distances between detection sites of identical individuals were relatively small or moderate in this study, suggesting regular movements of animals within their home ranges. Only two long-distance dispersal events exceeding 300 km were detected. Results of parental analysis provided evidence of pack distribution within the studied area. Most relationships were detected between wolves in the northern region of Czech Republic where the first recolonizing wolf pack in 2014 was registered. Within the studied...
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Population biology and evolutionary genetics of southern right whale (Eubalaena australis)
Nevečeřalová, Petra ; Hulva, Pavel (advisor) ; Galov, Ana (referee) ; Robovský, Jan (referee)
Southern right whale (Eubalaena australis) is a species from the Balaenidae family with a range in the southern hemisphere, which evolved in connection with Neogene climate oscillations. Its population biology involves migration between coastal waters of South America, southern Africa, Australia and New Zealand to its high-latitude foraging and feeding grounds in the Southern Ocean. Intensive historical hunting from the 18th to 19th century has impacted the population dynamics of baleen whales substantially. After the whaling moratorium, the species has been recovering from the heavy exploitation, however, recently it is facing anthropogenic changes, such as climate change. The South African population, considered to be the largest one globally and intensively studied for the last several decades, is showing an increase in the calving interval, a decline in female condition and a dramatic shift in migration routes since 2010, possibly as a consequence of significant changes in marine food webs. Methods of molecular ecology were applied to monitor the population genetics and its dynamics not only in the context of the global population but also to compare different time periods - 1990s and 2010s. The majority of the samples were collected noninvasively in cooperation with commercial whale-watching...
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Conservation genetics of the grey wolf in Central Europe
Valentová, Kamila Anna ; Hulva, Pavel (advisor) ; Galov, Ana (referee)
Conservation genetics of the grey wolf in Czech Republic and adjacent regions is studied in the present thesis. Analyses of twenty-one microsatellite loci, one sex-determining amelogenin gene and mitochodrial control region were used to verify species determination, identify individuals and estimate relationships between them, analyse population structure and estimate demographic trends based on samples collected between 2014 and 2021. Genetic detection of red fox and dog samples incorrectly assigned to wolves illustrates the hurdles of field monitoring of grey wolf. Direct evidence for the occurrence of F1 hybrids was not found. Wolves from Bohemia showed lower values of allelic richness in comparison to the ones from Western Carpathians, probably as a consequence of recent expansion. Geographic distances between detection sites of identical individuals were relatively small or moderate in this study, suggesting regular movements of animals within their home ranges. Only two long-distance dispersal events exceeding 300 km were detected. Results of parental analysis provided evidence of pack distribution within the studied area. Most relationships were detected between wolves in the northern region of Czech Republic where the first recolonizing wolf pack in 2014 was registered. Within the studied...
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