|
|
Mechanotransduction of Hepatic Cancer Cells cultured in a 3D Collagen Scaffold
Frtús, Adam ; Kubinová, Šárka (advisor) ; Filová, Elena (referee) ; Vištejnová, Lucie (referee)
Title: Mechanotransduction of Hepatic Cancer Cells cultured in a 3D Collagen Scaffold Author: Mgr. Adam Frtús Department: Department of Optical and Biophysical Systems, Institute of Physics of the Czech Academy of Sciences Supervisor: PharmDr. Šárka Kubinová, Ph.D., Department of Optical and Biophysical Systems, Institute of Physics of the Czech Academy of Sciences Abstract: Cell culture models transformed over time from a simple monolayer culture in 2D Petri dish to advanced 3D platforms, that provide conditions and features for cellular growth in all directions, similarly to in vivo. Moreover, cells experience distinct microenvironmental features within the extracellular matrix in tissues, that 3D cell culture might replicate. In fact, majority of studies indicate that physical cues generated from extracellular microenvironment drive the cellular behavior and functions. In this dissertation thesis, we attempt to provide the mechanistic explanation behind the changes in cellular metabolism and molecular signaling pathways induced by physical cues of 3D cell culture. We have used the advanced biomaterial-based 3D cell culture, biochemical assays, genetic manipulation and photobiomodulation in order to reveal the molecular mechanisms of mechanotransduction in hepatic cancer cells under physical cues of 3D...
|
guest ::
