|
| |
|
| |
|
|
The application of electrophoretic and chromatographic methods in clinical research
Fauknerová Matějčková, Jana ; Samcová, Eva (advisor) ; Pacáková, Věra (referee) ; Křepela, Evžen (referee)
- The application of electrophoretic and chromatographic methods in clinical research RNDr. Jana Fauknerová Matějčková Malondialdehyde (MDA) is considered to be the main biomarker for oxidative damage to biomembranes and an elevated level of this compound can act as an indicator for a number of diseases. I determined MDA using a HPLC method based on derivatization of blood plasma samples with 2,4-dinitrophenylhydrazine. I used HPLC to separate the MDA derivatives from the other components of the blood plasma and detected them at 307 nm. The MDA analysis time did not exceed 4.5 min. The sensitivity (detection limit 0.27 mol l-1 ) and repeatability of the determination of MDA are sufficient for monitoring the MDA level in real blood plasma samples. My work also describes monitoring the level of the low-molecular secondary antioxidant of uric acid, whose concentration in the blood attains values of 140 to 350 µmol l-1 and the secondary enzymatic antioxidant of superoxide dismutase (SOD). The uric acid level was analysed by the capillary electrophoresis and spectrophotometric methods, where the two methods yielded comparable results. SOD was analysed using a spectrophotometric kit. The MDA levels were measured for vegans (who refuse to eat any food of animal origin, meat, eggs and also dairy...
|
|
|
The application of electrophoretic and chromatographic methods in clinical research
Fauknerová Matějčková, Jana ; Samcová, Eva (advisor) ; Pacáková, Věra (referee) ; Křepela, Evžen (referee)
- The application of electrophoretic and chromatographic methods in clinical research RNDr. Jana Fauknerová Matějčková Malondialdehyde (MDA) is considered to be the main biomarker for oxidative damage to biomembranes and an elevated level of this compound can act as an indicator for a number of diseases. I determined MDA using a HPLC method based on derivatization of blood plasma samples with 2,4-dinitrophenylhydrazine. I used HPLC to separate the MDA derivatives from the other components of the blood plasma and detected them at 307 nm. The MDA analysis time did not exceed 4.5 min. The sensitivity (detection limit 0.27 mol l-1 ) and repeatability of the determination of MDA are sufficient for monitoring the MDA level in real blood plasma samples. My work also describes monitoring the level of the low-molecular secondary antioxidant of uric acid, whose concentration in the blood attains values of 140 to 350 µmol l-1 and the secondary enzymatic antioxidant of superoxide dismutase (SOD). The uric acid level was analysed by the capillary electrophoresis and spectrophotometric methods, where the two methods yielded comparable results. SOD was analysed using a spectrophotometric kit. The MDA levels were measured for vegans (who refuse to eat any food of animal origin, meat, eggs and also dairy...
|
guest ::
