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Phytochemical analysis and biological activity of the alga Haematococcus pluvialis and Chlorella sp.
Fábryová, Tereza ; Tůmová, Lenka (advisor) ; Mučaji, Pavel (referee) ; Dohnal, Vlastimil (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Department of Pharmacognosy Candidate Mgr. Tereza Fábryová Supervisor doc. PharmDr. Lenka Tůmová, PhD. Title of Doctoral Thesis Phytochemical analysis and biological activity of the alga Haematococcus pluvialis and Chlorella sp. Nowadays, microalgae represent a novel and promising source of various bio-active compounds. This research work focuses on two carotenoids - astaxanthin and lutein, which are attracting interest from various industrial sectors. However, the production methods so far developed for obtaining these two valuable carotenoid pigments from microalgae imply time- and solvent-consuming operations. This work deals with two aspects. Firstly, the investigation and development of efficient and scalable isolation methods for producing the target carotenoids from microalgae biomass using high-performance countercurrent chromatography (HPCCC). Secondly, the investigation of the biological activity of astaxanthin and its esters, which have been little studied. In our study, lutein was isolated from the green microalgae Chlorella vulgaris using the lower phase of the biphasic solvent system composed of n-heptane-ethanol-water (5:4:1.5, v/v/v) (LP4), which served both as a solvent for microalgae biomass extraction and as...
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