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Photosynthetic characteristics of potato plants with decreased expression of BEL11 transcription factor
Dybová, Magdaléna ; Mašková, Petra (advisor) ; Fischer, Lukáš (referee)
The potato (Solanum tuberosum) is one of the most important crops in the world. Tubers form during morphogenic process called tuberization, which timing is controlled by a complex regu- latory network involving both environmental conditions and internal factors. For the study of this process, model genotype of subspecies S. t. andigena is mostly used. It requires a short photoperiod. Vast majority of cultivated potatos are of spieces S. t. tuberosum that tuberize even under long-day conditions. Regulation of tuber initiation involves the action of endogenous factors, mainly phytohormones, carbohydrates and specific mobile signals, including BEL transcriptional factors - StBEL5, StBEL11 and StBEL29 - which transcripts are produced in leaves and transported into stolons. StBEL5 stimulates tuberization, whereas StBEL11 and -29 suppress it. The aim of this thesis was to contribute to understanding the role of StBEL11 in the regulation of tuberization by characterization of mutants with decreased StBEL11 levels. A sub- objective was to derive of transgenic lines S. t. andigena with decreased StBEL11 levels using a construct with part of StBEL11 coding region in antisense orientation under 35S CaMV pro- motor. I was able to derive forty two stable transgenic lines, which are prepared for StBEL11...
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