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Program-controlled freeze drying for the preparation of the drug-delivery system based on polysaccharide and hydrophobic molecules
Dufková, Michaela ; Kalina, Michal (referee) ; Enev, Vojtěch (advisor)
The lyophilization process favors the hydrophobic effect between the individual dextran chains. This hydrophobic effect allows hydrophobic drugs to settle and remain within the biopolymer. Due to hydrophobic effect, dextran can serve as a carrier for the distribution of hydrophobic drugs. The goal of this thesis was to prepare a carrier system based on native dextran using program-controlled freeze-drying. Hydrophobic drugs were modeled using the fluorescent probes pyrene and prodane. The effectiveness of promoting the hydrophobic effect was studied using infrared spectrometry, fluorescence spectrometry and scanning electron microscopy. Using ATR-FTIR spectrometry, a shift of the absorption band of thevalence vibration of the C–O bond in OH groups in alcohol was observed. Using fluorescence spectrometry, the emission polarity index was calculated for pyrene from the 1st and 3rd maximum fluorescence intensity, and for prodan, a shift of the maximum fluorescence intensity to lower wavelengths was observed. SEM images showed that during the lyophilization process, the dextran structure formed a dense network of fibers, indicating the presence of many sites with a higher content of hydrophobic domains. Positive results were obtained with these methods, which proved the positive effect of lyophilization on the formation of a hydrophobic effect between individual polysaccharide chains. The results may contribute to the development of carrier systems for hydrophobic drugs.
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Civil procedure in continental law and the Anglo-American legal system
Dufková, Michaela ; Macková, Alena (advisor) ; Pohl, Tomáš (referee)
The purpose of this thesis named 'Civil procedure in continental law and the Anglo- American legal system' is to provide an overview of the regulation of civil procedure in both mentioned systems, to compare them, highlight the most significant differences between them, and give the information about their convergence on international, mainly European level. The reason for the choice of this topic was my interest in the field of Civil Procedure, but also my belief in the benefit of comparisons of various legal orders in favour of improving them. Because of the on-going process of introducing uniform arrangements of some institutes of civil procedure on the European level, it is undoubtedly useful to compare national legislations in some respective countries. The research is based on study of legislation in Czech Republic and Germany as representatives of the continental system and in particular England and Wales as representatives of the Anglo-Saxon system. A shorter chapter is also devoted to U.S. law. There are aims of the thesis, methods of research and brief structure described in the introduction. The second chapter deals with the explanation of basics concepts necessary for proper understanding of the research. The third chapter is devoted to brief sketch of the history of civil procedure in...
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