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National Repository of Grey Literature 3 records found  Search took 0.00 seconds. 
Internalisation of anti-A?PP antibodies in cells.
 Doubková, Pavlína ; Hronek, Miloslav (advisor) ; Tilšer, Ivan (referee)
Introduction: Alzheimer disease (AD) is a specific type of dementia with a complex pathology. A formation of extracellular insoluble amyloid-beta (Aβ) fibrils from precursor protein (AβPP) has been identified as one of the main causes of AD. There are several enzymes involved in a production of Aβ; β-secretase has been recently considered as a potential target for AD treatment by methods of passive immunization. A monoclonal antibody (2B12) has been developed and proved that it binds in the vicinity of β-secretase cleavage site on AβPP and prevents the cleavage of AβPP by steric hindrance. 2B12 is known to binds to AβPP at the cell surface and the whole complex after internalization inhibits β- secretase activity. Methodology: The astrocytoma MOG-G-UVW (MOG) and the Human-CNS derived neuroglioma (H4) living cell lines were used as a model of AD. Incubated with 2B12, another AβPP - binding antibody (N-terminal) and several organelle markers (OM) under various conditions, the cells were fixed and stained by the method of sequential immunocytochemistry (ICC) and visualized using fluorescent microscope. Results: The experiments with MOG/H4 cells demonstrated that the intake of 2B12/N- terminal antibody into the cells is time-dependant; the best labelling was after 4 hours of incubation for 2B12 and MOG...
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Early age pertussis - the role of a nurse in prevention and treatment.
 DOUBKOVÁ, Pavlína
Abstract The aim of the thesis: To determine most common causes of pertussis in childhood is the aim of this submitted research thesis. It also describes the possibilities of child prevention from the point of view of nurses. The other aims are description the specifics of nursing care for children with pertussis. Five research questions have been defined in relations to these objectives. The way of achieving the aim: The qualitative research method was chosen to achieve the objectives of the thesis. This method was implemented through in-depth interviews. On the basis of identified research questions, individual categories have been formulated in order to get answers to individual questions. For selection of communication partners the method of intentional selection was chosen. Research was realized at Jihlava Hospital in Children's Department. Anonymous interviewing was attended by a total of 20 communication partners. All of them have a nursing education and have performed nursing care for a child with a pertussis disease. In-depth interviews with parents were carried out in General Practitioner for children and adoration. Anonymous survey were agreed by 7 parents whose children suffered from pertussis. Scientific benefits of the thesis: This research study deals with the current issue of pertussis as an omitted infection. The scientific results of the research are intended for both the lay and professional public. The research study presents a complex and comprehensive overview of nursing care for children with pertussis. It will find use in both preventive care and also in taking care of the patient directly on the bed. Part of the scientific thesis is application into practice. The lessons learned and conclusions: Diseases of pertussis are most at risk for newborns and infants, for whom their parents pose a significant threat of infection without the parents becoming aware of it. One of the main measures is to vaccinate not only children but also parents and close relatives who come to close contact with the newborn. Parents are often unaware of adult vaccination in the prevention of childhood illness. Nursing care for a child with pertussis disease has its specificities in that it is highly contagious illness and can endanger the life of the child.
Detailed record
Internalisation of anti-A?PP antibodies in cells.
 Doubková, Pavlína ; Tilšer, Ivan (referee) ; Hronek, Miloslav (advisor)
Introduction: Alzheimer disease (AD) is a specific type of dementia with a complex pathology. A formation of extracellular insoluble amyloid-beta (Aβ) fibrils from precursor protein (AβPP) has been identified as one of the main causes of AD. There are several enzymes involved in a production of Aβ; β-secretase has been recently considered as a potential target for AD treatment by methods of passive immunization. A monoclonal antibody (2B12) has been developed and proved that it binds in the vicinity of β-secretase cleavage site on AβPP and prevents the cleavage of AβPP by steric hindrance. 2B12 is known to binds to AβPP at the cell surface and the whole complex after internalization inhibits β- secretase activity. Methodology: The astrocytoma MOG-G-UVW (MOG) and the Human-CNS derived neuroglioma (H4) living cell lines were used as a model of AD. Incubated with 2B12, another AβPP - binding antibody (N-terminal) and several organelle markers (OM) under various conditions, the cells were fixed and stained by the method of sequential immunocytochemistry (ICC) and visualized using fluorescent microscope. Results: The experiments with MOG/H4 cells demonstrated that the intake of 2B12/N- terminal antibody into the cells is time-dependant; the best labelling was after 4 hours of incubation for 2B12 and MOG...
Detailed record

See also: similar author names
3 DOUBKOVÁ, Pavlína
1 Doubková, Pavla
2 Doubková, Petra
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