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Rapidly mutating Y-STRs analysis as a tool for forensic investigations, genetic genealogical applications and molecular anthropology
Jakub, David ; Šimková, Halina (advisor) ; Doubková, Klára (referee)
Compared to standard Y-STR markers commonly used in forensics and bioarchaeology, rapidly mutating Y-STR markers (RM Y-STR) have a significantly higher haplotype diversity and lineage resolution abilities in world populations. They form a novelty in these fields. This paper will attempt to clarify their origin, function, and efficacy in forensic genetics, genealogy, and molecular anthropology by looking at the Y chromosome and its characteristics that allow its use in the mapping of populations, individual male lineages, and identifying male perpetrators of sexual violence. By looking at other polymorphisms of the Y chromosome, especially Y-SNP markers, we can see in which studies each type is used most effectively. In the end, it will be clarified whether the use of RM Y-STR markers is beneficial to each field, whether they make standard Y-STR markers redundant, or whether they are themselves of no substantial use in comparison.
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Application of Capillary Electrophoresis in Forensic Molecular Genetic Investigation
Grafnetterová, Anna ; Doubková, Klára (advisor) ; Coufalová, Pavla (referee)
5 Abstract The capillary electrophoresis (CE) is an analytical method based on the principle of the traditional plate electrophoresis. Modern ways of fluorescence marking and the detection result in the higher reliability of analyses as well as in more precise results. The utilization of this method is wide within a lot of science fields. In special cases this method can even replace methods used so far, i.e. HPLC. The CE is mainly used in the forensic investigation in particular for person identification based on the DNA analysis of the even minimal sample amount. The STR analysis is an example of such examination. It uses short tandem repeats with high variability within the population. Commercial companies e.g. Promega Corporation, Applied Biosystems etc., develop corresponding instrumentation, software and kits which are important for receiving correct data. As a final result it is obtained DNA profile evaluated by comparison of samples from the "crime scene" with the reference samples. Besides the using in the human domain the DNA profiles are often used in the veterinary practice, in animal (cats, dogs, horses, cattle etc.) raising or breeding, for improving the particular characteristics. Key words: capillary electrophoresis fluorescent labeling STR STR analysis DNA profil
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Application of Capillary Electrophoresis in Forensic Molecular Genetic Investigation
Grafnetterová, Anna ; Doubková, Klára (advisor) ; Coufalová, Pavla (referee)
5 Abstract The capillary electrophoresis (CE) is an analytical method based on the principle of the traditional plate electrophoresis. Modern ways of fluorescence marking and the detection result in the higher reliability of analyses as well as in more precise results. The utilization of this method is wide within a lot of science fields. In special cases this method can even replace methods used so far, i.e. HPLC. The CE is mainly used in the forensic investigation in particular for person identification based on the DNA analysis of the even minimal sample amount. The STR analysis is an example of such examination. It uses short tandem repeats with high variability within the population. Commercial companies e.g. Promega Corporation, Applied Biosystems etc., develop corresponding instrumentation, software and kits which are important for receiving correct data. As a final result it is obtained DNA profile evaluated by comparison of samples from the "crime scene" with the reference samples. Besides the using in the human domain the DNA profiles are often used in the veterinary practice, in animal (cats, dogs, horses, cattle etc.) raising or breeding, for improving the particular characteristics. Key words: capillary electrophoresis fluorescent labeling STR STR analysis DNA profil
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Marketing research of customer care in the field of women's underwear
Doubková, Klára ; Horová, Olga (advisor) ; Konarovská, Helena (referee)
The bachelor thesis inquires into the customer care in the field of women's underwear and focuses primarily on helpfulness and sale skills of the staff, the specialized knowledge of the staff and the assortment of offered goods. The marketing research was based on the mystery shopping method in seven selected retail stores selling underwear. Seven independent instructed model customers participated in the mystery shopping. The research clearly shows that the staff employed in the stores is pleasant, but untrained, and the offered range of goods is definitely not able to satisfy all women. The situation is most critical in big chain stores.
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