Home > Search Results: Demydenko, Yana
Search:
Search Tips :: Advanced Search
Search collections:
Sort by: Display results: Output format:
National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Determination of lead in lung and brain samples of experimental mice after the inhalation of nanoparticles
 Demydenko, Yana ; Zlámalová Gargošová, Helena (referee) ; Vašinová Galiová, Michaela (advisor)
Lead is a heavy toxic metal whose nanoparticles are present in the air due to combustion processes. Data on the safe concentration of lead nanoparticles for human health have not been sufficiently investigated. Previous studies in experimental mice have shown that inhalation of lead nanoparticles first damages lungs and kidneys, then liver, spleen and brain. With prolonged exposure, lead accumulates in the teeth and bones. This bachelor thesis is focused on monitoring of the lead content in lung and brain samples of experimental mice after different periods of nanoparticle inhalation. The aim of this bachelor thesis was to develop an analytical method for the determination of lead in biological samples using atomic absorption spectrometry with electrothermal atomization (ET-AAS). In this work, a decomposition method for mineralization of the biological matrix in a microwave mineralizer was proposed, and a procedure for the determination of lead using ET-AAS was further developed. The determination of lead at 217 nm using the NH4H2PO4/Mg(NO3)2 matrix modifier was verified by analysis of certified reference materials. Analysis of organ samples from experimental mice confirmed the detoxification mechanism in exposed individuals with a dependence on the time since the end of inhalation of lead nanoparticles.
Detailed record
Determination of lead in lung and brain samples of experimental mice after the inhalation of nanoparticles
 Demydenko, Yana ; Zlámalová Gargošová, Helena (referee) ; Vašinová Galiová, Michaela (advisor)
Lead is a heavy toxic metal whose nanoparticles are present in the air due to combustion processes. Data on the safe concentration of lead nanoparticles for human health have not been sufficiently investigated. Previous studies in experimental mice have shown that inhalation of lead nanoparticles first damages lungs and kidneys, then liver, spleen and brain. With prolonged exposure, lead accumulates in the teeth and bones. This bachelor thesis is focused on monitoring of the lead content in lung and brain samples of experimental mice after different periods of nanoparticle inhalation. The aim of this bachelor thesis was to develop an analytical method for the determination of lead in biological samples using atomic absorption spectrometry with electrothermal atomization (ET-AAS). In this work, a decomposition method for mineralization of the biological matrix in a microwave mineralizer was proposed, and a procedure for the determination of lead using ET-AAS was further developed. The determination of lead at 217 nm using the NH4H2PO4/Mg(NO3)2 matrix modifier was verified by analysis of certified reference materials. Analysis of organ samples from experimental mice confirmed the detoxification mechanism in exposed individuals with a dependence on the time since the end of inhalation of lead nanoparticles.
Detailed record

Interested in being notified about new results for this query?
Subscribe to the RSS feed.
Digital Repository :: Search :: Submit :: Personalize :: Help
Powered by Invenio v1.1.2
Maintained by admin.invenio@techlib.cz
This site is also available in the following languages:
Česky  English