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The effect of methylviologen on secondary metabolites production in in vitro culture of Hypericum perforatum
Chreňová, Kateřina ; Tůmová, Lenka (advisor) ; Kašparová, Marie (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmacognosy Candidate: Mgr. Kateřina Chreňová Consultant: doc. PharmDr. Lenka Tůmová, CSc. Title of Thesis: The effect of methyl viologen on secondary metabolites production in in vitro culture of Hypericum perforatum The study examined the effect of methyl viologen on production of secondary metabolites in callus and suspension cultures of Hypericum perforatum L. The monitored secondary metabolites were hyperosid, quercetin and hypericin. For elicitation was used methyl viologen in three concentrations: 3.6341.10-5 mol l-1 ; 3.6341.10-4 mol l-1 a 3.6341.10-3 mol l-1 . As a nutrien medium was chosen MS medium with addition of growth regulator 10 g l-1 α-NAA (α-naphtylacetic acid). Cultivation with the elicitor lasted for 6, 12, 24, 48, 72 and 168 hours. The samples of dry weight and medium was analyzed by HPLC. The highest hyperosid production was detected after elicitor treatment in concentration of 3.6341.10-4 mol l-1 after 24 and 168 hours in suspension culture. Another efficient production was detected after elicitor aplication in concentration of 3.6341.10-3 mol l-1 in suspension culture. Production of quercetin was not so significant. Hyperosid and quercetin were released into a nutrient medium of callus and suspension...
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The effect of methylviologen on secondary metabolites production in in vitro culture of Hypericum perforatum
Chreňová, Kateřina ; Tůmová, Lenka (advisor) ; Kašparová, Marie (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmacognosy Candidate: Mgr. Kateřina Chreňová Consultant: doc. PharmDr. Lenka Tůmová, CSc. Title of Thesis: The effect of methyl viologen on secondary metabolites production in in vitro culture of Hypericum perforatum The study examined the effect of methyl viologen on production of secondary metabolites in callus and suspension cultures of Hypericum perforatum L. The monitored secondary metabolites were hyperosid, quercetin and hypericin. For elicitation was used methyl viologen in three concentrations: 3.6341.10-5 mol l-1 ; 3.6341.10-4 mol l-1 a 3.6341.10-3 mol l-1 . As a nutrien medium was chosen MS medium with addition of growth regulator 10 g l-1 α-NAA (α-naphtylacetic acid). Cultivation with the elicitor lasted for 6, 12, 24, 48, 72 and 168 hours. The samples of dry weight and medium was analyzed by HPLC. The highest hyperosid production was detected after elicitor treatment in concentration of 3.6341.10-4 mol l-1 after 24 and 168 hours in suspension culture. Another efficient production was detected after elicitor aplication in concentration of 3.6341.10-3 mol l-1 in suspension culture. Production of quercetin was not so significant. Hyperosid and quercetin were released into a nutrient medium of callus and suspension...
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In vitro cultures of medicinal plants XX
Chreňová, Kateřina ; Tůmová, Lenka (advisor) ; Kašparová, Marie (referee)
The study is aimed to enhance in vitro production of secondary metabolites in Genista tinctoria L. via elicitor treatment. The different levels of elicitor concentration - selenium dioxide were utilized to affect the quantity of isoflavonoids occurred in cultures. Experiment was perfomed in callus and suspension cultures on MS nutrient media supplemented with 10 g l-1 of NAA (α-naphtylacetic acid) as growth regulator. The elicitor was added in the form of solution in concentrations of 9,012.10-3 mol l-1 ; 9,012.10-4 mol l-1 and 9,012.10-5 mol l-1 . It was exposed for 6, 12, 24, 48, 72 and 168 hours. The content of isoflavonoids was determined by HPLC in dry weight (DW) and medium. The most effective production of genistin (6,20 mg. g-1 DW, 8,30 mg. g-1 DW) in callus culture was measured. It was reached in concentrations of 9,012.10-4 mol l-1 and 9,012.10-5 mol l-1 after 168 h elicitor treatment. The second most satisfactory genistin level 5,20 mg g-1 DW was detected after elicitor application in concentration of 9,012.10-4 mol l-1 after 6 h. The content of genistein, daidzein and formononetin in callus culture was low and in the most cases equal zero compared to control samples. The content of biochanin A was equal zero compared to control samples. The most efficient daidzein production (37,10 mg...
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