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Influence of needle age at Rubisco activity and content in Norway spruce under the impact of ambient and elevated CO2 concentrations
Bošková, Martina ; Vítová, Eva (referee) ; Hrstka, Miroslav (advisor)
In this diploma work influence of needle age at Rubisco activity and content in Norway spruce (Picea abies) was studied. The plants were cultivated in conditions with ambient (A) CO2 concentration (350 µmol CO2/mol) and elevated (E) CO2 concentration (700 µmol CO2/mol). Sampling was done two times during the growing season (in the middle of June and in the end of September) were taken. Initial and total Rubisco activities were measured spectrophotometrically. Rubisco content was determined by SDS–PAGE method. Rubisco activity in 18-months-old needles was in E higher than in A. Rubisco contents in current-year needles and one-year-old needles were in A higher than in E in September. These differences were statistically significant that demonstrates the down-regulation of Rubisco content in conditions of elevated CO2 concentration. It seems the course of activities and content depending on age of the needles are antiparallel, that means that decrease of content is followed by increase of activity.
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Influence of needle age at Rubisco activity and content in Norway spruce under the impact of ambient and elevated CO2 concentrations
Bošková, Martina ; Vítová, Eva (referee) ; Hrstka, Miroslav (advisor)
In this diploma work influence of needle age at Rubisco activity and content in Norway spruce (Picea abies) was studied. The plants were cultivated in conditions with ambient (A) CO2 concentration (350 µmol CO2/mol) and elevated (E) CO2 concentration (700 µmol CO2/mol). Sampling was done two times during the growing season (in the middle of June and in the end of September) were taken. Initial and total Rubisco activities were measured spectrophotometrically. Rubisco content was determined by SDS–PAGE method. Rubisco activity in 18-months-old needles was in E higher than in A. Rubisco contents in current-year needles and one-year-old needles were in A higher than in E in September. These differences were statistically significant that demonstrates the down-regulation of Rubisco content in conditions of elevated CO2 concentration. It seems the course of activities and content depending on age of the needles are antiparallel, that means that decrease of content is followed by increase of activity.
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Cultural heritage in the development of municipalities and regions on the example of the region of South Bohemia
Bošková, Martina ; Kadeřábková, Jaroslava (advisor) ; Khendriche Trhlínová, Zuzana (referee)
This thesis deals with cultural and natural heritage in the region of South Bohemia, in particular the relationship of cultural and natural heritage for tourism and joint action on the development of the region. The theoretical part is characterized by a general culture, the cultural and natural heritage, heritage conservation legislation and its financing in the Czech Republic, cultural tourism. It deals with the relationship of heritage conservation and tourism. The practical part of this work assesses the current state of cultural and natural heritage and exploit the potential for the development of tourism in the region. Finds the conditions for its further development and provides for limiting factors of development.
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