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The use of supercritical fluid chromatography in pharmaceutical analysis
Babičová, Barbora ; Khalikova, Maria (advisor) ; Matysová, Ludmila (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Barbora Babičová Supervisor: Mgr. Maria Khalikova, CSc. Consultant: doc. PharmDr. Lucie Nováková, Ph.D. Title of the Diploma Thesis: The use of supercritical fluid chromatography in pharmaceutical analysis Keywords: enzalutamide, ticagrelor, supercritical fluid chromatography, method validation Enzalutamide is a new drug for the treatment of metastatic form of prostate cancer. Ticagrelor is a drug with antiplatelet activity. The optimization of UHPSFC/UV methods for analysis of both active substances enzalutamide, and ticagrelor, and their respective impurities is described in diploma thesis. In the case of enzalutamide, there were 4 impurities analysed using following conditions: Viridis BEH 2-EP column (3.0 x 100 mm, 1.7 µm) with a column temperature of 40 řC. The mobile phase used was methanol with 10 mM ammonium formate and gradient elution using 0-40 % of organic mobile phase modifier over 3 minutes with a flow rate of 1.5 ml/min. The UV detection was proceed at 270 nm. The total analysis time was 4.5 minutes. Separation of ticagrelor and its 2 impurities was made using Acquity UPLC HSS C18 SB column (3.0 x 100 mm, 1.8 µm). The mobile phase was composed of methanol and acetonitrile (1:1,...
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