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Strukturní studie halogenalkandehalogenasy DgaA z \kur{Glaciecola agarilytica} NO2
BERKOVÁ, Ivana
Haloalkane dehalogenases (HLDs) are microbial enzymes that have attracted significant interest because of their ability to catalyze the irreversible hydrolysis of a wide range of halogenated compounds. These enzymes can be used as potential applicants in industrial catalysis, in the bioremediation and the biosensing of environmental pollutants. Novel haloalkane dehalogenase DgaA (EC 3.8.1.5, HLDs) belonging to the superfamily of / hydrolases, was isolated from a psychrophilic and moderately halophilic organism, Glaciecola agarilytica NO2, that was found in marine sediment collected from the East Sea, Korea. Main target of this thesis was the processing of diffraction data from crystals of DgaA proteins and subsequent solving and refinement of structure of studied protein.
Krystalizační studie nově izolované halogenalkandehalogenasy DgaA z \kur{Glacieocola agarilytica} NO2
BERKOVÁ, Ivana
This bachelors thesis is focused on crystalization study of newly prepared halogenalkanedehalogenase DgaA from bacteria Glaciecola agarylitica NO2. Main target of this work is getting acquianted with methods of protein crystalization and usage of those methods for preparation of suitable DgaA protein crystals of that will be used for X-ray structural analysis. Results from difraction analysis of DgaA crystals will be starting point for further research focused on structure determination and and description of protein function.

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3 BERKOVÁ, Ilona
2 BERKOVÁ, Iva
1 Berková, Irena
2 Berková, Iva
1 Berková, Ivona
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