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Aplication of circulating and disseminated tumor cells detection in the treatment and diagnosis of solid tumors
Eliášová, Petra ; Bobek, Vladimír (advisor) ; Adámek, Svatopluk (referee) ; Svobodová, Pavla (referee)
Introduction: Circulating tumor cells (CTC) and disseminated tumor cells (DTC) are responsible for the development of metastasis. Detection of CTC from peripheral blood (so- called "liquid biopsy") may contribute to the diagnosis and choice of therapy in patients in whom it is not possible to obtain tissue directly from the tumor. Monitoring the effect of surgical treatment and detecting early recurrence of cancer are other potential uses for CTC. Aim: The aim of our work was to isolate CTC/DTC from blood or peritoneal lavage of patients with various types of solid tumors and then try to cultivate them in vitro and to describe their cytomorphological characteristics. We targeted the following hypotheses: I. Do CTCs occur in different types of solid tumors? II. Does the presence of CTC reflect cancer stage? III. Is it possible to use CTC for molecular characterization of cancer? IV. Is it possible to visualize CTC/DTC in a viable status? Methods: CTC/DTC detection was performed in a total of 288 patients, including 24 patients with pancreatic cancer, 165 with breast cancer, 98 with colorectal cancer and 1 with a neuroendocrine tumor of the small intestine. A cell size separation protocol (MetaCell®) was used to enrich CTC. After simple filtration of blood through a polycarbonate membrane with pores,...
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The diagnosis and surgical treatment of primary hyperparathyroidism
Libánský, Petr ; Adámek, Svatopluk (advisor) ; Marek, Josef (referee) ; Haruštiak, Svetozár (referee)
Surgical management of primary hyperparathyroidism is a very effective method. The target is to cure primary hyperparathyroidism and to reach normal calcium levels. This results in an improvement of health condition and resolution or at least moderation of symptoms. Complications are infrequent and mortality is very low. Surgical management is definite, safe and effective. Number, location and surrounding structures of parathyroid glands with emphasis on parathyroidectomy were studied on 101 cadaverous bodies. We recommend the performance of parathyroidectomy in a circumscribed area 2 cm in diameter, 1 cm cranially to the intersection of the inferior thyroid artery and recurrent laryngeal nerve. We identified 75% of samples as parathyroid glands. The rest of collected samples were lymph nodes, fat particles and thyroid or lipothymic tissue. During the course of this study, we have tested techniques of miniinvazive procedures. In the following part, the bony, metabolic and biochemical syndromes are evaluated. We presented series of 151 patients that have been operated with the diagnosis of primary hyperparathyroidism. The survey is focused on the primary hyperparathyroidism and concomitant diseases and on the possible sequelae in the postoperative period. The special part is describing 10 clinical...
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The diagnosis and surgical treatment of primary hyperparathyroidism
Libánský, Petr ; Adámek, Svatopluk (advisor) ; Marek, Josef (referee) ; Haruštiak, Svetozár (referee)
Surgical management of primary hyperparathyroidism is a very effective method. The target is to cure primary hyperparathyroidism and to reach normal calcium levels. This results in an improvement of health condition and resolution or at least moderation of symptoms. Complications are infrequent and mortality is very low. Surgical management is definite, safe and effective. Number, location and surrounding structures of parathyroid glands with emphasis on parathyroidectomy were studied on 101 cadaverous bodies. We recommend the performance of parathyroidectomy in a circumscribed area 2 cm in diameter, 1 cm cranially to the intersection of the inferior thyroid artery and recurrent laryngeal nerve. We identified 75% of samples as parathyroid glands. The rest of collected samples were lymph nodes, fat particles and thyroid or lipothymic tissue. During the course of this study, we have tested techniques of miniinvazive procedures. In the following part, the bony, metabolic and biochemical syndromes are evaluated. We presented series of 151 patients that have been operated with the diagnosis of primary hyperparathyroidism. The survey is focused on the primary hyperparathyroidism and concomitant diseases and on the possible sequelae in the postoperative period. The special part is describing 10 clinical...
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