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Roman spa - sauna world
Šupová, Veronika ; Myslín, Jiří (referee) ; Dýr, Petr (advisor)
Architectural study offers solution space of Roman baths and sauna world in designed Aquacenter , which is located in Brno - Rakovec area. Location aqua center study is based on the Territorial studies Brněnská přehrada area. Nowadays wellness facilities are important part of water parks. The designed spaces of Roman baths and sauna world offer its visitors the use Roman baths, Finnish saunas, as well as other supporting procedures such as massages, herbal baths, massage whirlpools, etc. It is not just a place to stay the recovery or relaxation, but is included here social function as generously solved frigidarium space s and relaxing bar. Form, which is based on the design building of Aquacenter, is circular and it allows central layout solution. The central solution dispositions are reflected throughout the interior design concept. The overall layout is also trying to build on traditional content Roman bath, respect the Finnish sauna concept and both join together in a common area.
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Isolation of bacterial flagellin as an immunogen for the preparation of antibodies for cystic fibrosis patients
Supová, Veronika ; Hodek, Petr (advisor) ; Kubíčková, Božena (referee)
Cystic fibrosis (CF) is an autosomal recessive disease caused by CFTR gene mutation. The consequence of this mutation is an improper function of the chloride channel consisting of CFTR protein. Due to this abnormality, the transport of chloride ions is either reduced or inhibited completely, which leads to a mucus secretion. Mucus is mostly created in the lungs and it is the ideal environment for pathogenic bacteria like Pseudomonas aeruginosa (PA). Pseudomonas aeruginosa is an aerobic, gram-negative conditioned pathogen occurring in patients with weakened immunity such as patients with CF, who are often hospitalized. PA has one polar flagellum which contains filament composed of a protein called flagellin. The flagellum is one of the most important virulence factors of PA bacteria. This thesis focused on the isolation of flagellin from Pseudomonas aeruginosa flagella. The isolated flagellin will serve as an antigen for the preparation of prophylactic antibodies for CF patients. The isolation of flagellin was carried out by four methods using combinations of precipitation and centrifugation. The published methods were optimized to achieve the isolation of flagellin. Final samples and intermediates were analyzed by SDS-electrophoresis on polyacrylamide gel and the presence of flagellin was verified...
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Roman spa - sauna world
Šupová, Veronika ; Myslín, Jiří (referee) ; Dýr, Petr (advisor)
Architectural study offers solution space of Roman baths and sauna world in designed Aquacenter , which is located in Brno - Rakovec area. Location aqua center study is based on the Territorial studies Brněnská přehrada area. Nowadays wellness facilities are important part of water parks. The designed spaces of Roman baths and sauna world offer its visitors the use Roman baths, Finnish saunas, as well as other supporting procedures such as massages, herbal baths, massage whirlpools, etc. It is not just a place to stay the recovery or relaxation, but is included here social function as generously solved frigidarium space s and relaxing bar. Form, which is based on the design building of Aquacenter, is circular and it allows central layout solution. The central solution dispositions are reflected throughout the interior design concept. The overall layout is also trying to build on traditional content Roman bath, respect the Finnish sauna concept and both join together in a common area.
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