|
|
Study of plasma interaction with bacteria for wound healing
Šrámková, Sarah ; Krčma, František (referee) ; Kozáková, Zdenka (advisor)
This thesis focuses on the study of the interaction of plasma with bacteria to enhance the promotion of wound healing. Firstly, the wound healing process is described, followed by low temperature plasma, its effect on bacterial cells and its use in biomedicine. The experimental part deals with the application of low-temperature plasma generated by microwave jet on selected microorganisms and the influence of experimental conditions on the antimicrobial effect of the plasma. One representative of the microorganisms occurring in the wounds was selected as representatives of Gram-positive bacteria and Gram-negative bacteria, namely Staphylococcus epidermidis and Escherichia coli. Using the results obtained, the antimicrobial effect of plasma generated by microwave jet in argon was confirmed. The degree of antimicrobial effect is related to the concentration of the microorganisms, the time of treatment and whether the bacteria are Gram-positive or Gram-negative.
|
|
|
Application of plasma and plasma activates water for wound healing
Šrámková, Sarah ; Janda,, Mário (referee) ; Kozáková, Zdenka (advisor)
This work is focused on the study of plasma interactions with liquids and selected microorganisms, Staphylococcus epidermidis and Escherichia coli. The use of the cold atmospheric pressure plasma in the biomedical field has been a hot topic in the last decade, due to its decontamination properties applicable not only in the biomedical field but also in the food industry and agriculture. Recently, the plasma treated (activated) liquids (PTLs) have also been a subject of a great interest due to the relatively simple preparation, the possibility of optimizing the composition of PTLs to achieve the desired effect, their non-toxic nature and their environmental friendliness. The purpose of this work is to study the inhibitory effects of the plasma discharges and the plasma treated liquids for the purpose of decontamination and stimulation of wound healing. To study the effect of a direct plasma application on selected microorganisms, two types of discharges, the unipolar microwave torch and the microwave surface wave jet, were selected. For both selected discharges, the dependence of the inhibitory effects on the treatment time, the power, the closure of the treated area and the bacterial species (gramnegative/grampositive) was studied. Comparable results in the inhibition efficiency were obtained for both discharges. The effect of indirect treatment using plasma-treated water was studied next. A decent microbial reduction occurred for all three studied PTLs. The PTL prepared using a plasma jet with a direct current (DC) source was found to be the most efficient. The last stated goal of this diploma thesis was to study the effect of treatment combinig both the indirect treatment with PTL and the direct plasma treatment. Based on the results of previous treatments, a bacterial culture of Staphylococcus epidermidis was treated with PTL prepared with a direct current (DC) plasma jet. The unipolar microwave torch with a power of 12 W was selected for the direct treatment. By combining both types of treatments, higher inhibition effeciency was achieved. The inhibition efficiency was demonstrated for all types of treatments, with the combined treatment aspiring to be the most effective, as it targets both grampositive and gramnegative bacteria.
|
|
|
Study of plasma interaction with bacteria for wound healing
Šrámková, Sarah ; Krčma, František (referee) ; Kozáková, Zdenka (advisor)
This thesis focuses on the study of the interaction of plasma with bacteria to enhance the promotion of wound healing. Firstly, the wound healing process is described, followed by low temperature plasma, its effect on bacterial cells and its use in biomedicine. The experimental part deals with the application of low-temperature plasma generated by microwave jet on selected microorganisms and the influence of experimental conditions on the antimicrobial effect of the plasma. One representative of the microorganisms occurring in the wounds was selected as representatives of Gram-positive bacteria and Gram-negative bacteria, namely Staphylococcus epidermidis and Escherichia coli. Using the results obtained, the antimicrobial effect of plasma generated by microwave jet in argon was confirmed. The degree of antimicrobial effect is related to the concentration of the microorganisms, the time of treatment and whether the bacteria are Gram-positive or Gram-negative.
|
guest ::
RSS feed.