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The design of eye-lens simulator
Špaček, Matěj ; Rebenda, David (referee) ; Nečas, David (advisor)
The aim of this bachelor thesis is a design of an eye-lens simulator. Tribometer module in pin-on-ring configuration was designed to allow direct observation of a contact zone. The survey of existing tribometers was conducted as a part of the thesis, based on which, three concept solutions were suggested. Final solution of the module was chosen and designed based on the concepts. The apparatus enables to simulate conditions of real human eye so it could contribute to a contact lens research and eye lubrication solution development.
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Design of manipulator of the sample for CCHM microscope
Špaček, Matěj ; Brada, Michal (referee) ; Dostál, Zbyněk (advisor)
In the Laboratory of the Experimental Biophotonics at Brno University of Technology, a new generation of the Coherence-Controlled Holographic Microscope (CCHM) is being developed. Because of the automation of certain microscope procedures, a fully motorized three axis computer controlled manipulator of the sample is to be employed. A fast and accurate lateral positioning in a range of several centimeters is required. For the focusing capability, very precise positioning in the optical axes is required as well. Initially, the basics of microscope manipulator design are presented. Then, commercially available solutions are introduced and compared with special focus on its possible implementation in the new generation of CCHM. However, due to the specific design of the new CCHM, available manipulators are not suitable. Therefore, a new optimized manipulator design is introduced in the second part of the thesis. A selection of its appropriate elements, especially actuators, is based on a review. For the new manipulator design, a technical documentation was made and a testing software was developed. A crucial part of the manipulator was assembled and tested.
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Design of the imaging part of the coherence-controlled holographic microscope
Dvořák, Vladislav ; Dostál, Zbyněk (referee) ; Špaček, Matěj (advisor)
The Experimental Biophotonics Research group at Brno University of Technology has been involved in the development of Coherence controlled holographic microscope (CCHM). For the purposes of further development in this field, it is necessary to build an open laboratory set-up of the microscope, which has only been available in a commercial design. The objective of this thesis is to design and assemble its imaging unit. The first part of the thesis is dedicated to a general overview of microscopic methods, with an emphasis on interference microscopy and principle of CCHM microscope. In the second part, different optical set-up concepts are discussed and compared. Then, the optical components are selected and the unknown parameters of these components are experimentally measured. With the use of measured parameters, the optical tracing and mechanical design are introduced. The imaging unit has been assembled based on the suggested design and experimentally verified.
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Design of illumination module for CCHM microscope
Pachl, Přemysl ; Špaček, Matěj (referee) ; Dostál, Zbyněk (advisor)
This bachelor thesis starts with a brief overview of the basic imaging methods used in classical and quantitative phase microscopy. The main aim of the thesis is to design, construct and test a tomographic illuminator compatible with the laboratory version of the Q-PHASE microscope. The design is decomposed into several parts, where in the first step the optical requirements of the system are determined using the laws of geometrical optics. Suitable optical components are then selected and simulations are performed in the Zemax software to verify that all specified requirements are met. The compactness and compatibility of the assembly is taken into account in the design. Therefore, a prism with total internal reflection is used to illuminate the amplitude light modulator. Furthermore, the design solutions for mounting the light source, the prism and the amplitude modulator are proposed. The developed drawings are attached to this thesis. The whole assembly is assembled, adjusted and its correct function is verified by means of 16 test tomographic projections.
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Design of manipulator of the sample for CCHM microscope
Špaček, Matěj ; Brada, Michal (referee) ; Dostál, Zbyněk (advisor)
In the Laboratory of the Experimental Biophotonics at Brno University of Technology, a new generation of the Coherence-Controlled Holographic Microscope (CCHM) is being developed. Because of the automation of certain microscope procedures, a fully motorized three axis computer controlled manipulator of the sample is to be employed. A fast and accurate lateral positioning in a range of several centimeters is required. For the focusing capability, very precise positioning in the optical axes is required as well. Initially, the basics of microscope manipulator design are presented. Then, commercially available solutions are introduced and compared with special focus on its possible implementation in the new generation of CCHM. However, due to the specific design of the new CCHM, available manipulators are not suitable. Therefore, a new optimized manipulator design is introduced in the second part of the thesis. A selection of its appropriate elements, especially actuators, is based on a review. For the new manipulator design, a technical documentation was made and a testing software was developed. A crucial part of the manipulator was assembled and tested.
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The design of eye-lens simulator
Špaček, Matěj ; Rebenda, David (referee) ; Nečas, David (advisor)
The aim of this bachelor thesis is a design of an eye-lens simulator. Tribometer module in pin-on-ring configuration was designed to allow direct observation of a contact zone. The survey of existing tribometers was conducted as a part of the thesis, based on which, three concept solutions were suggested. Final solution of the module was chosen and designed based on the concepts. The apparatus enables to simulate conditions of real human eye so it could contribute to a contact lens research and eye lubrication solution development.
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