|
|
Molecular and epidemiological characteristics of Burkholderia cenocepacia strain dominant in cystic fibrosis patients in the Czech Republic
Vilimovská Dědečková, Klára ; Dřevínek, Pavel (advisor) ; Macek, Milan (referee) ; Šmajs, David (referee)
Summary: Bacteria from BurkJwlderia cepacia complex represent the most serious pathogens for patients witb cystic fibrosis. l nc:ir scverity IS caused by their antibiotic resistance and ability to Sllrcad among patients wilh cystic fibrosis. Many cyslic fibrosis cenlrcs around IlIe world, also tbe biggest Czech Cystic Fibrosis Centre in University Hospital in Motol, faeed outbreak. In Ihe 1990's tlle epidernie strain HurMwlderia cenocepacia S1'32 spread in a population of cystic fibrosis patienlS. One of the goals of the tunent thesis was to study epidemiological situation of Blďkholderia cepada oornplex in the popolation of eystie fibrosis pmients allending Cysfie Fibrosis Centre in University Hospilal MOlol almost seventeen years after first cases of infected palients wilh Bec inrection. Otber goals were: to evaloate 3 usefulness of molccular genelie diagnosties of Burkholderia cepacia complex tetl years afier ils implementation in routine diagnosties, to improve surveillal1ee syslem not only by implementalion or simple and specifie tesl for detection of epidemie stmin, bul 3150 by implementation of genolyping melllods thal are able to detennine strain in olher cases of infeetion. The [asI goal was to characterize Ibe epidemie slrain frolll virolence point ofview. Dased oRthe thesis we: confirmed...
|
|
| |
|
|
Genetic basis of multidrug resistance in Acinetobacter baumannii
Křížová, Lenka ; Nemec, Alexandr (advisor) ; Higgins, Paul (referee) ; Šmajs, David (referee)
Charles University in Prague, Faculty of Science Department of Genetics and Microbiology Ph.D. study program: Molecular and Cellular Biology, Genetics and Virology Genetic basis of multidrug resistance in Acinetobacter baumannii Lenka Křížová Supervisor: Doc. RNDr. Alexandr Nemec, Ph.D. Supervisor-consultant: RNDr. Lubomír Janda, Ph.D. Prague 2014 SUMMARY Acinetobacter baumannii has emerged as a significant bacterial pathogen pre-eminently associated with hospital-acquired infections. Strains of this species may currently exhibit resistance to nearly all or even all clinically relevant drugs. The vast majority of epidemic and multidrug-resistant A. baumannii strains belong to a few globally spread lineages, in particular to the so-called European (EU) clones I, II, and III. Complex resistance patterns displayed by these strains result from their marked capacity to develop, acquire, and combine secondary resistance mechanisms against originally effective agents. The aim of this thesis was to broaden our knowledge on the genetic basis and epidemiology of multidrug resistance in A. baumannii. The obtained results have been published in the form of six studies which are part of this thesis. In the first study, we analysed the epidemiology of carbapenem resistance among hospital strains of Acinetobacter in the...
|
|
|
Molecular and epidemiological characteristics of Burkholderia cenocepacia strain dominant in cystic fibrosis patients in the Czech Republic
Vilimovská Dědečková, Klára ; Dřevínek, Pavel (advisor) ; Macek, Milan (referee) ; Šmajs, David (referee)
Summary: Bacteria from BurkJwlderia cepacia complex represent the most serious pathogens for patients witb cystic fibrosis. l nc:ir scverity IS caused by their antibiotic resistance and ability to Sllrcad among patients wilh cystic fibrosis. Many cyslic fibrosis cenlrcs around IlIe world, also tbe biggest Czech Cystic Fibrosis Centre in University Hospital in Motol, faeed outbreak. In Ihe 1990's tlle epidernie strain HurMwlderia cenocepacia S1'32 spread in a population of cystic fibrosis patienlS. One of the goals of the tunent thesis was to study epidemiological situation of Blďkholderia cepada oornplex in the popolation of eystie fibrosis pmients allending Cysfie Fibrosis Centre in University Hospilal MOlol almost seventeen years after first cases of infected palients wilh Bec inrection. Otber goals were: to evaloate 3 usefulness of molccular genelie diagnosties of Burkholderia cepacia complex tetl years afier ils implementation in routine diagnosties, to improve surveillal1ee syslem not only by implementalion or simple and specifie tesl for detection of epidemie stmin, bul 3150 by implementation of genolyping melllods thal are able to detennine strain in olher cases of infeetion. The [asI goal was to characterize Ibe epidemie slrain frolll virolence point ofview. Dased oRthe thesis we: confirmed...
|
|
| |
|
| |
|
| |
guest ::
