National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Characterization of Euglena gracilis and their role in metaboly movement
Šmída, Adam ; Hampl, Vladimír (advisor) ; Pergner, Jiří (referee)
Euglena gracilis is a free-living freshwater flagellate from the class Euglenida (Euglenozoa, Discoba, Excavata). The synapomorphy of Euglenida group is their pellicle, a firm layer underneath the surface of the cell which is composed of proteinaceous stripes (pellicle strips). The strips are locked into each other and run along the cell`s surface underneath the plasma membrane. In E. gracilis these strips can move by parallel sliding, therefore contracting the cell and changing its surface which results in a characteristic peristaltic euglenoid movement called metaboly. The mechanism behind this movement is still unresolved, however, it is expected that the force is generated by the cytoskeletal structures beneath the pellicle strips which consist of microtubules and other unknown cytoskeletal fibers. This thesis tests a hypothesis which proposes that centrins are involved in the pellicle contraction. Centrins are small proteins capable of binding calcium ions, they are very conserved throughout the eukaryotic tree and serve many purposes in the cell, of which one is to form contractile structures in ciliates species e.g., Vorticella or Paramecium. Sequences of centrins were searched for in the transcriptome of E. gracilis and after comparison with the available proteomic dataset of the isolated...
Silicification of diatoms from a cellular, evolutional and ecological point of view
Šmída, Adam ; Kulichová, Jana (advisor) ; Němcová, Yvonne (referee)
Diatoms are a significant protist group. They've been getting a lot of attention because of their various silica cell walls since their discovery at the end of the 18th century. Even though the mechanism of the morphologically variable cell wall synthesis is well known in terms of shape and ornamentation, only recently it has been enabled to investigate the diatom silicification on the molecular basis. The knowledge of the silica cell wall synthesis can then be used to understand general acpects of biosilica handling and to enlighten the possible evolutionary substance and the progress of this phenomenon. Key words: biomineralisation, diatoms, long chain polyamins, nanostructure, silaffins, silica transporters, silica deposit vesicle, silicification

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