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Synthesis of fluorescent polyene ceramide
Škarda, Filip ; Vávrová, Kateřina (advisor) ; Roh, Jaroslav (referee)
Filip Škarda Synthesis of fluorescent polyene ceramides Charles University in Prague, Faculty of Pharmacy in Hradec Králové 2014 Ceramides are lipid compounds that belong to the sphingolipid family. These molecules play a crucial role in many biological processes e.g. they are secondary messengers during apoptosis and other cellular signaling pathways, are involved in the synthesis of sphingomyelin and play very important role in the lipid matrix of stratum corneum, which is the epidermal barrier. Recent results showed that ceramides are affecting, or are being affected by various skin diseases. In the case of atopic dermatitis, a major decrease of ceramides in the stratum corneum is observed. On the other hand, the manifestation of psoriasis or atopic dermatitis can be reduced with topically administered ceramides. The topical ceramides repair the skin lipid barrier and prevent further water loss and aggravation of the disease. The mechanism of action of topically administered ceramides has not been fully elucidated yet. This is one of the reasons why the selection of the right methodology in order to clarify the mechanism of action is crucial. One of the possibilities is the introduction of a fluorophore into the ceramide molecule. Currently, various fluorophores (NBD, Bodipy, Rhodamine) are used...
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Synthesis of fluorescent polyene ceramide
Škarda, Filip ; Vávrová, Kateřina (advisor) ; Roh, Jaroslav (referee)
Filip Škarda Synthesis of fluorescent polyene ceramides Charles University in Prague, Faculty of Pharmacy in Hradec Králové 2014 Ceramides are lipid compounds that belong to the sphingolipid family. These molecules play a crucial role in many biological processes e.g. they are secondary messengers during apoptosis and other cellular signaling pathways, are involved in the synthesis of sphingomyelin and play very important role in the lipid matrix of stratum corneum, which is the epidermal barrier. Recent results showed that ceramides are affecting, or are being affected by various skin diseases. In the case of atopic dermatitis, a major decrease of ceramides in the stratum corneum is observed. On the other hand, the manifestation of psoriasis or atopic dermatitis can be reduced with topically administered ceramides. The topical ceramides repair the skin lipid barrier and prevent further water loss and aggravation of the disease. The mechanism of action of topically administered ceramides has not been fully elucidated yet. This is one of the reasons why the selection of the right methodology in order to clarify the mechanism of action is crucial. One of the possibilities is the introduction of a fluorophore into the ceramide molecule. Currently, various fluorophores (NBD, Bodipy, Rhodamine) are used...
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Opportunities of Using Open Data for Competitive Intelligence
Škarda, Filip ; Molnár, Zdeněk (advisor) ; Kučera, Jan (referee)
This thesis discusses the opportunities of using open data particularly from the perspective of commercial organizations. At first, the concept of open data is generally described including the relationships with related concepts. The thesis also mentions the general benefits and estimated economic impact of using open data. Next, it is described the current situation of open data activities in the Czech republic, the current possible sources of open data and also the possible business models for open data. The practical benefits of this thesis include the visualized outputs from the content analysis of national open data catalogues data.gov.uk and govdata.de, which demonstrate what kind of published datasets are available in those catalogues as an example for the Czech republic and also the conclusions of the performed pre-survey on demand for open data in the Czech republic. Based on this and similar surveys around the world, it was found that there is a big interest on open data from the commercial organizations and the biggest issue is its insufficient availability.
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One Time Passwords
Škarda, Filip ; Pavlíček, Luboš (advisor) ; Palovský, Radomír (referee)
This Bachelor thesis describes the one-time passwords and their various implementation possibilities. The one-time passwords are passwords, which are valid only for one login or transaction. In contrast to regular passwords, one-time passwords are immune against reply attacks, which means that if a possible attacker eavedroppes the used password, he cannot exploit it, because it is not valid anymore. The theoretical section of the thesis explains the reasons for using one-time passwords and demonstrates some particular OTP systems. The practical section shows two different examples of using open-source OTP systems. The first one describes the use of OTPasswd for authentication to a server over SSH, the other one uses MultiOTP PHP class for authentication to a web application.
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