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Study of bioaccessibility and oxidative potential of elements using simulated lung fluids
Šidla, Jiří ; Doležalová Weissmannová, Helena (referee) ; Cigánková,, Hana (advisor)
This master thesis deals with the determination of the bioaccessible concentration and oxidative potential of an urban aerosol using 3 simulated lung fluids. Submicron fraction (PM1; urban aerosol particles with aerodynamic diameter less than 1 m) was collected in two campaigns (summer, winter). The mass concentration of PM1 was determined gravimetrically and total concentrations of selected metals were determined by ICP-MS. Next, extraction of the sampled filters into 3 simulated lung fluids (Gamble's solution – GS, Simulated alveoli fluid – SAF, and Curosurf) was performed. The extracts were used to determine the bioaccessibility of each metal by ICP-MS and to determine the oxidative potential. Oxidative potential of standard solutions of selected metals and real aerosol samples was also studied using 3 simulated lung fluids. The highest total concentration in PM1 was measured for Fe. The bioaccessibility of most of the studied metals was higher in Curosurf than in GS and SAF. Although the bioaccessibility of most metals was higher in the summer campaign than in the winter campaign, higher values of OP were measured in the winter campaign. OP was higher for extracted PM in Curosurf than in SAF. A study of relationships between measured variables was also conducted using correlations. This work provides a closer look at the effects of inhaled metals contained in urban atmospheric aerosol on human health. The inclusion of Curosurf among the SLFs analysed is also beneficial, as it is a substance used in hospitals for the treatment of respiratory distress syndrome in children.
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Monitoring the activity of selected groups of skeletal muscles during squats on balance pads using EMG.
Šidla, Jiří ; Bedáňová, Kamila (referee) ; Adamík, Richard (advisor)
This bachelor thesis is focused on the topic of monitoring the activity of selected groups of skeletal muscles during squats on balance pads. Thigh muscles of 21 probands (21,81 ± 2,06 years old, 184,53 ± 5,19 cm, 72,50 ± 4,75 kg) were measured using EMG when they did squats without balance pad and with five balance pads (BOSU concavely, BOSU convexly, T-bow, foam pad and Vicore). Ten of these persons was measured also with inflatable balance pod. Mean and mean of the 10 highest peaks of filtered and normalised measured signals were statistically analysed. There were some statistic differences of both parametres between measurement without balance pad and measurements with some balance pads (BOSU concavely, BOSU convexly, Vicore and balance pod) using analysis of all thigh muscles together, analysis of anterior-posterior stability and analysis of dexter-sinister stability. There were some statistic differences between these balance pads and the other two pads (T-bow, foam pad).
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Monitoring the activity of selected groups of skeletal muscles during squats on balance pads using EMG.
Šidla, Jiří ; Bedáňová, Kamila (referee) ; Adamík, Richard (advisor)
This bachelor thesis is focused on the topic of monitoring the activity of selected groups of skeletal muscles during squats on balance pads. Thigh muscles of 21 probands (21,81 ± 2,06 years old, 184,53 ± 5,19 cm, 72,50 ± 4,75 kg) were measured using EMG when they did squats without balance pad and with five balance pads (BOSU concavely, BOSU convexly, T-bow, foam pad and Vicore). Ten of these persons was measured also with inflatable balance pod. Mean and mean of the 10 highest peaks of filtered and normalised measured signals were statistically analysed. There were some statistic differences of both parametres between measurement without balance pad and measurements with some balance pads (BOSU concavely, BOSU convexly, Vicore and balance pod) using analysis of all thigh muscles together, analysis of anterior-posterior stability and analysis of dexter-sinister stability. There were some statistic differences between these balance pads and the other two pads (T-bow, foam pad).
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