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Isolation of vacuoles from Scutellaria baicalensis Georgii suspension cultures
Ševčíková, Šárka ; Martin, Jan (advisor) ; Dušek, Jaroslav (referee)
10. ABSTRACT Šárka Ševčíková Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Heyrovského 1203, 500 05 Hradec Králové Isolation of vacuoles from Scutellaria baicalensis Georgii suspension cultures Diploma thesis Supervisor: Pharm.Dr. Jan Martin, Ph.D. Page Count: 52 The goal of the thesis was to develop methodology for isolation of vacuoles from suspension cultures of Scutellaria baicalensis Georgii of the Lamiaceae family. The original concept was based on a method used at the Division of Pharmacognosy, Leiden University, Holland. This method proved unsuitable. After getting acquainted with available papers concerning methods of isolation of vacuoles from miscellaneous plant species and after performing a series of experiments, we have managed to introduce such method of our own which utilizes Ficoll gradient. Preexisting methods of isolation of vacuoles varied in data on lytic enzymes, incubation time, lytic solution composition and density gradient, too. We managed to optimize the individual steps, which led to successful isolation of vacuoles. The resulting method makes it possible to isolate vacuoles in a relatively short time and in sufficient quality for further experiments. Keywords: Baikal Skullcap, Scutellaria, vacuoles, flavonoids, ficoll, gradient, isolation of vacuoles
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Project of centralization operating theatres in Hospital Děčín
Ševčíková, Šárka ; Trpišovský, Milan (advisor) ; Rejholec, Jan (referee)
This diploma paper deals with a business plan processing for the operating theatres centralization in Hospital Děčín. In the desk study I summed up knowledge from the sphere of strategic management, business plan creation, marketing and I put there also the description of surgical tract including building layout and personal constitution. In the empirical part there is the business plan of operating rooms' centralization and the detailed description of following chapters: description of organization, product, marketing plan, management plan, operating plan, financial plan, legal plan. The description of organization covers its features, resent achievements, history and present of surgery in Hospital Děčín. The chapter product is divided into two bodies. The first body attends to the characterization of contemporary product - decentralized surgeries including the numbers of performed operations over the past three years. The second part is all about the new product (centralized operation theatres) and its specification. There are figured out anticipated variable expenses, fixed costs and profit. In the marketing plan, which is first of all focused on internal clients, you can find structural analysis, marketing concept and goals, marketing strategy with the definition of marketing mix and competitive...
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Project of centralization operating theatres in Hospital Děčín
Ševčíková, Šárka ; Trpišovský, Milan (advisor) ; Rejholec, Jan (referee)
This diploma paper deals with a business plan processing for the operating theatres centralization in Hospital Děčín. In the desk study I summed up knowledge from the sphere of strategic management, business plan creation, marketing and I put there also the description of surgical tract including building layout and personal constitution. In the empirical part there is the business plan of operating rooms' centralization and the detailed description of following chapters: description of organization, product, marketing plan, management plan, operating plan, financial plan, legal plan. The description of organization covers its features, resent achievements, history and present of surgery in Hospital Děčín. The chapter product is divided into two bodies. The first body attends to the characterization of contemporary product - decentralized surgeries including the numbers of performed operations over the past three years. The second part is all about the new product (centralized operation theatres) and its specification. There are figured out anticipated variable expenses, fixed costs and profit. In the marketing plan, which is first of all focused on internal clients, you can find structural analysis, marketing concept and goals, marketing strategy with the definition of marketing mix and competitive...
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Isolation of vacuoles from Scutellaria baicalensis Georgii suspension cultures
Ševčíková, Šárka ; Martin, Jan (advisor) ; Dušek, Jaroslav (referee)
10. ABSTRACT Šárka Ševčíková Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Heyrovského 1203, 500 05 Hradec Králové Isolation of vacuoles from Scutellaria baicalensis Georgii suspension cultures Diploma thesis Supervisor: Pharm.Dr. Jan Martin, Ph.D. Page Count: 52 The goal of the thesis was to develop methodology for isolation of vacuoles from suspension cultures of Scutellaria baicalensis Georgii of the Lamiaceae family. The original concept was based on a method used at the Division of Pharmacognosy, Leiden University, Holland. This method proved unsuitable. After getting acquainted with available papers concerning methods of isolation of vacuoles from miscellaneous plant species and after performing a series of experiments, we have managed to introduce such method of our own which utilizes Ficoll gradient. Preexisting methods of isolation of vacuoles varied in data on lytic enzymes, incubation time, lytic solution composition and density gradient, too. We managed to optimize the individual steps, which led to successful isolation of vacuoles. The resulting method makes it possible to isolate vacuoles in a relatively short time and in sufficient quality for further experiments. Keywords: Baikal Skullcap, Scutellaria, vacuoles, flavonoids, ficoll, gradient, isolation of vacuoles
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