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Biomarkers of neurodegeneration in serum and cerebrospinal fluid in patients with selected neurological diseases
Nosková, Libuše ; Fialová, Lenka (advisor) ; Tlaskalová - Hogenová, Helena (referee) ; Švarcová, Jana (referee)
Neurofilaments are the key structural component of the cytoskeleton of neurons, where they are essential for many functions. They consist of 3 subunits: light chain (NFL); medium chain (NfM) and heavy chain (NfH). Except neurofilament proteins there is also α-internexin in the central nervous system (CNS) or peripherin in the peripheral NS. Due to various pathophysiological processes, neurofilament proteins are released into the extracellular space, where they can interact with the components of the immune system. While the involvement of the immune system in the pathogenesis of neurodegenerative diseases is obvious, less knowledge about the antibody response to the neurofilament proteins is available. It is eligible to expand our knowledge in this area. Determination of free antibodies against neurofilaments together with their immune complexes with corresponding antigen provides us more detailed insight into the antibody immune response against neurofilaments. We have optimized the ELISA methods to determine free antibodies against light and heavy chain of neurofilaments together with their corresponding immunocomplexes in both serum and cerebrospinal fluid. Implementation of these methods is precondition for analysis of those parameters in serum and cerebrospinal fluid of patients with...
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Příčiny eliminace koní ve vytrvalostních soutěžích a jejich prevence
ŠVARCOVÁ, Jana
The aim of this work was to acquire, process and evaluate information about the most common reasons for the elimination of horses from endurance competition races in the Czech Republic. Information about the veterinary status of horses was obtained from the main judges of various horse races held in the Czech Republic, from the horses' endurance cards, which record the factors evaluated during the competition itself, and where it is possible to find out what worsens most often, and how the cause manifests itself. Veterinary cards of individual horses and races were chosen randomly. The veterinary cards were evaluated with the aid of a full text commentary, giving detailed descriptions of each worsened factor, including their frequency. It was then suggested how problems could be avoided. Individual data was summarized, and the differences between individual groups of competitions were expressed. Based on the analysis of veterinary cards from participating horses in endurance competitions, recommendations were made on how to prevent problems, and what specific problems the riders should consider during the race. The most serious and common problems of horses in terms of endurance are described. It was found that the most common reason for the worsening of horses in endurance races is the factor known in Czech as "Chody", or Lameness. The most frequent deteriorating factor in veterinary controls was with the Mucous Membrane. In more than 80% of horses, more than two factors worsened during the race, which did not improve during the race in any way, but often even worsened. Many factors affect deterioration, from training and preparation of the horse, to the knowledge of the horse itself. The results show that most riders in the Czech Republic are not sufficiently informed. There is insufficient care for horses during endurance races. The riders are not experienced enough to prevent injuries or deterioration of the horse's health resulting in exclusion from races.
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Anti-cytoskeletal antibodies in patients with multiple sclerosis and other neurological diseases
Švarcová, Jana
This thesis focuses on the role of anti-cytoskeletal antibodies in various neurodegenerative diseases. Autoantibodies against different axonal cytoskeletal proteins, such as the light (NF-L) and medium (NF-M) subunits of neurofilament and tubulin (TU), in serum and cerebrospinal fluid may be generated in response to the release of cytoskeleton from damaged neurons. The aim of this study was to assess autoimmune involvement in amyotrophic lateral sclerosis (ALS) as well as to evaluate antibody light and medium neurofilament subunit in serum and cerebrospinal fluid (CSF) of patients with ALS. Furthermore, we were interested in the relationships among these antibodies in the serum and in the CSF as well as between the two anti-NF antibody subtypes. Secondly, the aim was to compare the levels of anti-tubulin antibodies (anti-TU) in cerebrospinal fluid and serum in multiple sclerosis (MS) disease, using bovine tubulin as the antigen in one enzyme-linked immunosorbent assay (ELISA) method (anti-TUb antibodies) and a synthetic neuron-specific octapeptide of tubulin in a second ELISA method (anti-TUs antibodies). In the observed groups of ALS patients, serum levels of anti-NF-L antibodies were higher in ALS patients than in controls and serum anti-NF-L antibodies and intrathecal anti- NF-M antibodies were...
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Anti-cytoskeletal antibodies in patients with multiple sclerosis and other neurological diseases
Švarcová, Jana ; Matouš-Malbohan, Ivan (advisor) ; Tlaskalová - Hogenová, Helena (referee) ; Průša, Richard (referee)
This thesis focuses on the role of anti-cytoskeletal antibodies in various neurodegenerative diseases. Autoantibodies against different axonal cytoskeletal proteins, such as the light (NF-L) and medium (NF-M) subunits of neurofilament and tubulin (TU), in serum and cerebrospinal fluid may be generated in response to the release of cytoskeleton from damaged neurons. The aim of this study was to assess autoimmune involvement in amyotrophic lateral sclerosis (ALS) as well as to evaluate antibody light and medium neurofilament subunit in serum and cerebrospinal fluid (CSF) of patients with ALS. Furthermore, we were interested in the relationships among these antibodies in the serum and in the CSF as well as between the two anti-NF antibody subtypes. Secondly, the aim was to compare the levels of anti-tubulin antibodies (anti-TU) in cerebrospinal fluid and serum in multiple sclerosis (MS) disease, using bovine tubulin as the antigen in one enzyme-linked immunosorbent assay (ELISA) method (anti-TUb antibodies) and a synthetic neuron-specific octapeptide of tubulin in a second ELISA method (anti-TUs antibodies). In the observed groups of ALS patients, serum levels of anti-NF-L antibodies were higher in ALS patients than in controls and serum anti-NF-L antibodies and intrathecal anti- NF-M antibodies were...
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Trénování koní pro vytrvalostní sport
ŠVARCOVÁ, Jana
The aim of this work was to process information about endurance riding in the Czech Republic and draw recommendations based on the analysis of a sample of horses competing in endurance. A questionnaire was made to obtain information about the training of horses. It was sent to higher level riders willing to provide information about training process of their horses. Necessary requirement for filling the questionnaire was a horse that competed in at least 60 km competition level. The questionnaire was made individually with each rider and contains questions regarding training, horse management, feeding, other influences on the horse etc. Data from the questionnaire were complemented with a full text commentary indicating detailed process of each horse's preparation. Individual data were summarized and differences between individual groups were assessed. It was found out that the highest participation, superior to other CEN races by almost 90 %, is in competitions between 40 and 79 km. Horses without verified origin or Shagya Arabians were used most often. For the given load horses must be slowly prepared by training. Training, overall fitness, and other factors are influenced by feeding and rider's abilities as well. It is obvious from the results that it is impossible to build one training plan for all horses. It is possible to create uniform, clear overview of training schedules though and rider can afterwards modify individual parts of training depending on abilities and the training level of his horse.
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Anti-cytoskeletal antibodies in patients with multiple sclerosis and other neurological diseases
Švarcová, Jana
This thesis focuses on the role of anti-cytoskeletal antibodies in various neurodegenerative diseases. Autoantibodies against different axonal cytoskeletal proteins, such as the light (NF-L) and medium (NF-M) subunits of neurofilament and tubulin (TU), in serum and cerebrospinal fluid may be generated in response to the release of cytoskeleton from damaged neurons. The aim of this study was to assess autoimmune involvement in amyotrophic lateral sclerosis (ALS) as well as to evaluate antibody light and medium neurofilament subunit in serum and cerebrospinal fluid (CSF) of patients with ALS. Furthermore, we were interested in the relationships among these antibodies in the serum and in the CSF as well as between the two anti-NF antibody subtypes. Secondly, the aim was to compare the levels of anti-tubulin antibodies (anti-TU) in cerebrospinal fluid and serum in multiple sclerosis (MS) disease, using bovine tubulin as the antigen in one enzyme-linked immunosorbent assay (ELISA) method (anti-TUb antibodies) and a synthetic neuron-specific octapeptide of tubulin in a second ELISA method (anti-TUs antibodies). In the observed groups of ALS patients, serum levels of anti-NF-L antibodies were higher in ALS patients than in controls and serum anti-NF-L antibodies and intrathecal anti- NF-M antibodies were...
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Anti-cytoskeletal antibodies in patients with multiple sclerosis and other neurological diseases
Švarcová, Jana ; Matouš-Malbohan, Ivan (advisor) ; Tlaskalová - Hogenová, Helena (referee) ; Průša, Richard (referee)
This thesis focuses on the role of anti-cytoskeletal antibodies in various neurodegenerative diseases. Autoantibodies against different axonal cytoskeletal proteins, such as the light (NF-L) and medium (NF-M) subunits of neurofilament and tubulin (TU), in serum and cerebrospinal fluid may be generated in response to the release of cytoskeleton from damaged neurons. The aim of this study was to assess autoimmune involvement in amyotrophic lateral sclerosis (ALS) as well as to evaluate antibody light and medium neurofilament subunit in serum and cerebrospinal fluid (CSF) of patients with ALS. Furthermore, we were interested in the relationships among these antibodies in the serum and in the CSF as well as between the two anti-NF antibody subtypes. Secondly, the aim was to compare the levels of anti-tubulin antibodies (anti-TU) in cerebrospinal fluid and serum in multiple sclerosis (MS) disease, using bovine tubulin as the antigen in one enzyme-linked immunosorbent assay (ELISA) method (anti-TUb antibodies) and a synthetic neuron-specific octapeptide of tubulin in a second ELISA method (anti-TUs antibodies). In the observed groups of ALS patients, serum levels of anti-NF-L antibodies were higher in ALS patients than in controls and serum anti-NF-L antibodies and intrathecal anti- NF-M antibodies were...
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The effectiveness of the environmental policies of the European Union
ŠVARCOVÁ, Jana
The thesis defines the contemporary attitude of the European union to the environment and a way of a reflection of this attitude to its economic policy. The author of the thesis aims to contribute to the discussion and real effectiveness to such a young policy. The thesis contains example studies reflecting the basic principles of the European union and its relation to the environment as well as the instruments of the implemenation of these principles. A list of economic instruments of the environmental policy is worked up there. The sustainable development as one of the main contemporary objectives of the European union and respect of it are strongly emphasised there. The thesis outlines the final picture of the policy which is still influenced by profit and non-profit subjects. Answers to the following questions should be a result of the thesis: What is the real effectiveness of the environmental policy of the European union? Does the European union tends to the sustainable development or does it tends to the economic development?
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Tourism trade and its environment
ŠVARCOVÁ, Jana
Work deal with region Horšovskotýnsko and tourism in this area. Aim of work was valorize state of tourism in this area and create suggestion that would here improved situation. Proposal become cycling trace.
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