|
|
Možnosti aplikace mastných kyselin při ukončení alkoholové fermentace
Černoch, Michal
This bachelor thesis deals with the issue of higher medium chain fatty acids (MCFA) and their effect on the termination of alcoholic fermentation. The literature describes fatty acids, carbonyl compounds, alcoholic fermentation and sulfur dioxide in general. In the experimental part, an experiment was carried out in which MCFA was applied and the effect on the end of fermentation and the content of carbonyl compounds was observed. The experiment was divided into two application variants. The main difference was that variant A was the applied MCFA to the wine lees, and wine was racked after three days, variant B then consisted in the application of MCFA immediately after racking of the wine. Chemical and sensory analysis was performered then. The effectiveness of MCFA in inhibiting microorganism was confirmed by chemical analysis. Within the total carbonyl compounds, the content was lower in variant B than in variant A but the difference was negligible. Compared to wines produced by standard technology, the wines where MCFA was applied the content was rapidly lower. Sensory analysis has shown that the recommended dose of MCFA does not affect organoleptic properties. The wines were fruity, with tones of citrus and tropical fruits.
|
|
|
Role of RecQ helicases in maintenance of genomic stability during mitosis
Černoch, Marek ; Janščák, Pavel (advisor) ; Půta, František (referee)
Helicases are proteins capable of unwinding nucleic acids, their malfunction can be dangerous for genome stability of the cell. Five RecQ-family helicases identified in human cells participate in many cellular events during the whole cell cycle, including mitosis, and therefore are very important for correct functioning. The mutations in RecQ helicases can cause them to malfunction and seriously damage various cell processes, for example DNA replication, DNA damage control or sister chromatids separation. The mutations can also lead to dangerous syndromes, with the hallmark symptom of increased risk of cancer.
|
|
|
The study of epigenetic regulation of HLA class II genes at the level of histone modification
Černoch, Marek ; Černá, Marie (advisor) ; Hirsch, Ivan (referee)
Introduction: The epigenetic modifications can significantly affect and alter the gene activity by regulating their expression, having direct impact on various processes in human body. Epigenetic processes are involved in ethiopathogenesis of many diseases. From this point of view, MHC genes are very important as they were linked to many autoimmune disorders, for example type 1 diabetes mellitus. In general autoimmune diseases appear to be connected to certain MHC class II genes. Aims: The aim of this thesis is to determine the relationship between expression levels and histone modifications present in the promoter area of MHC class II gene, DQA1. Moreover, we also analyze and compare the DQA1 gene mRNA expression depending on the QAP promoter allele. Methods: We isolated both nucleic acids (DNA and RNA) and leukocytes from peripheral blood samples collected from voluntary donors. DNA was utilized for genotypization of individuals. RNA was subjected to reverse transcription and the quantitative PCR was performed in order to determine the level of expression. Leukocytes were used for chromatin immunoprecipitation, which was evaluated using quantitative PCR. Results: The expression level of QAP allele 3.1 was found to be higher than for the rest of the alleles Allele 4.1A showed, on the other hand,...
|
|
|
Role of RecQ helicases in maintenance of genomic stability during mitosis
Černoch, Marek ; Janščák, Pavel (advisor) ; Půta, František (referee)
Helicases are proteins capable of unwinding nucleic acids, their malfunction can be dangerous for genome stability of the cell. Five RecQ-family helicases identified in human cells participate in many cellular events during the whole cell cycle, including mitosis, and therefore are very important for correct functioning. The mutations in RecQ helicases can cause them to malfunction and seriously damage various cell processes, for example DNA replication, DNA damage control or sister chromatids separation. The mutations can also lead to dangerous syndromes, with the hallmark symptom of increased risk of cancer.
|
|
|
Leadership of Virtual Teams
Černoch, Michal ; Kašparová, Eva (advisor) ; Surynek, Alois (referee)
This thesis contemplates virtual teams, in particular the role of a leader in them. In the theoretical part of the thesis I explore and describe specific characteristics of virtual teams that distinguish them from the traditional teamwork paradigm, benefits and risks stemming from their use in the business world and policies and principles of leadership of different types of virtual teams that help achieve their maximum efficiency. In the practical portion of the thesis I demonstrate the negative effects of neglecting those principles by studying an example of a real, failed virtual team.
|
|
| |
|
| |
|
| |
|
| |
|
| |
guest ::
RSS feed.